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Abnormal expression of chondroitin sulfate sulfotransferases in the articular cartilage of pediatric patients with Kashin-Beck disease.
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2019 (English)In: Histochemistry and Cell Biology, ISSN 0948-6143, E-ISSN 1432-119XArticle in journal (Refereed) Epub ahead of print
Abstract [en]

The objective of this study is to investigate the expression of enzymes involved in the sulfation of articular cartilage from proximal metacarpophalangeal (PMC) joint cartilage and distal metacarpophalangeal (DMC) joint cartilage in children with Kashin-Beck disease (KBD). The finger cartilage samples of PMC and DMC were collected from KBD and normal children aged 5-14 years old. Hematoxylin and eosin staining as well as immunohistochemical staining were used to observe the morphology and quantitate the expression of carbohydrate sulfotransferase 3 (CHST-3), carbohydrate sulfotransferase 12 (CHST-12), carbohydrate sulfotransferase 13 (CHST-13), uronyl 2-O-sulfotransferase (UST), and aggrecan. In the results, the numbers of chondrocyte decreased in all three zones of PMC and DMC in the KBD group. Less positive staining cells for CHST-3, CHST-12, CHST-13, UST, and aggrecan were observed in almost all three zones of PMC and DMC in KBD. The positive staining cell rates of CHST-12 were higher in superficial and middle zones of PMC and DMC in KBD, and a significantly higher rate of CHST-13 was observed only in superficial zone of PMC in KBD. In conclusion, the abnormal expression of chondroitin sulfate sulfotransferases in chondrocytes of KBD children may provide an explanation for the cartilage damage, and provide therapeutic targets for the treatment.

Place, publisher, year, edition, pages
2019.
Keywords [en]
Children’s cartilage, Chondroitin sulfate, Kashin–Beck disease, Selenium deficiency, Sulfation, Sulfotransferases
National Category
Rheumatology and Autoimmunity Cell and Molecular Biology Biochemistry and Molecular Biology
Research subject
molecular medicine (medical sciences); rheumatology
Identifiers
URN: urn:nbn:se:umu:diva-166647DOI: 10.1007/s00418-019-01833-0PubMedID: 31845005OAI: oai:DiVA.org:umu-166647DiVA, id: diva2:1380832
Available from: 2019-12-19 Created: 2019-12-19 Last updated: 2020-01-09

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Lammi, Mikko

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Lammi, MikkoQu, Chengjuan
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Department of Integrative Medical Biology (IMB)
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Histochemistry and Cell Biology
Rheumatology and AutoimmunityCell and Molecular BiologyBiochemistry and Molecular Biology

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