umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Hyaluronan and the receptor CD 44 in the heart and vessels: a study in normal and pathological conditions
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine, Medicine. Umeå University, Faculty of Medicine, Department of Clinical Sciences, Otorhinolaryngology.
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Tissues are not solely composed of cells. The extracellular matrix is important for the cell well-being and cell-cell communication. The glycosaminoglycan hyaluronan (HYA) is a widely distributed extracellular matrix (ECM) component. The molecule has prominent physicochemical properties, foremost viscoelastic and osmotic, but participates in many biological processes such as cell migration, proliferation, tissue turnover, wound healing and angiogenesis. HYA is synthesised by either of three different hyaluronan-synthesising enzymes, HAS1-3, and its main ligand is the transmembrane receptor CD44. In the heart and vessels the matrix components are of great importance for endurance and elasticity which are prerequisites for a normal function. The aims of the study were to describe the distribution of HYA and its receptor CD44 in normal cardiovascular tissue and to investigate the ECM composition in myocardial hypertrophy.

Normal conditions were studied in a rat model. These studies showed that the tunica adventitia in almost all vessels stained strongly for HYA. The expression in the tunica intima and media on the venous side, differed between the vessels and was almost absent on the arterial side. In the adult animals only minute amounts of CD44 were detected. The expression of both HYA and CD44 was increased in newborn rats.

In the heart HYA was unevenly distributed in the interstitium. Strong HYA-staining was seen in the valves and in the adventitia of intramyocardial vessels. Almost no CD44-staining was observed. Notably, there was no obvious difference between newborn and adult animals.

In an experimental rat model of pressure-induced cardiac hypertrophy the mRNA-levels of HAS1, HAS2, CD44, basic Fibroblast Growth Factor (FGF-2) and Fibroblast Growth Factor Receptor-1 (FGFR-1) were elevated on day 1 after aortic banding. HAS2, CD44 and FGFR-1 were at basal levels on day 42. The HYA-concentration was significally elevated on day 1. HYA was detected in the interstitium by histochemistry and CD44 was detected mainly in and around the intramyocardial vessels.

The HYA-staining was increased in myectomi specimens from patients with HCM compared to controls. HYA was detected in the interstitium, in fibrous septas and in the adventitia of intramyocardial vessels. No CD44 was detected in HCM or in control specimens.

Our results indicate that HYA and CD44 play an active role in the maturing vessel tree and that the ECM content of HYA is increased in experimental myocardial hypertrophy and human hypertrophic cardiomyopathy.

Place, publisher, year, edition, pages
Umeå: Folkhälsa och klinisk medicin , 2007. , 53 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1104
Keyword [en]
hyaluronan, CD44, heart, Rat, cardiac hypertrophy, artery, vein, human
Research subject
Medicine
Identifiers
URN: urn:nbn:se:umu:diva-1128ISBN: 978-91-7264-324-6 (print)OAI: oai:DiVA.org:umu-1128DiVA: diva2:140298
Public defence
2007-06-01, Sal B, Tandläkarhögskolan, 9tr, Norrlands universitets sjukhus, Umeå, 09:00 (English)
Opponent
Supervisors
Available from: 2007-05-10 Created: 2007-05-10 Last updated: 2011-04-08Bibliographically approved
List of papers
1. Hyaluronan is differently located in arteries and veins: an immunohistochemical study in the rat
Open this publication in new window or tab >>Hyaluronan is differently located in arteries and veins: an immunohistochemical study in the rat
2003 (English)In: Cells Tissues Organs, ISSN 1422-6405, Vol. 173, no 4, 227-233 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-2339 (URN)10.1159/000070378 (DOI)12766352 (PubMedID)
Available from: 2007-05-10 Created: 2007-05-10Bibliographically approved
2. Expression of the CD44 receptor in the blood vessel system: an experimental study in rat.
Open this publication in new window or tab >>Expression of the CD44 receptor in the blood vessel system: an experimental study in rat.
2005 (English)In: Cells Tissues Organs, ISSN 1422-6405, Vol. 179, no 3, 102-108 p.Article in journal (Refereed) Published
Keyword
Aging/blood, Animals, Animals; Newborn, Antigens; CD44/analysis/*biosynthesis, Arteries/chemistry/metabolism, Blood Vessels/chemistry/*metabolism, Hyaluronic Acid/analysis, Immunohistochemistry, Male, Rats, Rats; Sprague-Dawley, Veins/chemistry/metabolism
Identifiers
urn:nbn:se:umu:diva-14570 (URN)doi:10.1159/000085001 (DOI)15947460 (PubMedID)
Available from: 2008-01-11 Created: 2008-01-11 Last updated: 2009-09-22Bibliographically approved
3. Hyaluronan and its receptor CD44 in the heart of newborn and adult rats.
Open this publication in new window or tab >>Hyaluronan and its receptor CD44 in the heart of newborn and adult rats.
2006 (English)In: Anatomical record part A: discoveries in molecular, cellular, and evolutionary biology, ISSN 1552-4884, Vol. 288, no 6, 587-592 p.Article in journal (Refereed) Published
Keyword
Aging, Animals, Animals; Newborn, Antigens; CD44/*analysis, Aorta/chemistry/metabolism, Aortic Valve/chemistry/metabolism, Coronary Vessels/chemistry/metabolism, Heart Atria/chemistry/metabolism, Heart Ventricles/chemistry/metabolism, Hyaluronic Acid/*analysis, Immunohistochemistry, Male, Myocardium/*chemistry/metabolism, Pulmonary Artery/chemistry/metabolism, Pulmonary Valve/chemistry/metabolism, Rats, Rats; Sprague-Dawley
Identifiers
urn:nbn:se:umu:diva-15343 (URN)doi:10.1002/ar.a.20332 (DOI)16700028 (PubMedID)
Available from: 2007-07-04 Created: 2007-07-04 Last updated: 2011-08-24Bibliographically approved
4. Parallel up-regulation of FGF-2 and hyaluronan during development of cardiac hypertrophy in rat
Open this publication in new window or tab >>Parallel up-regulation of FGF-2 and hyaluronan during development of cardiac hypertrophy in rat
Show others...
2008 (English)In: Cell and Tissue Research, ISSN 0302-766X, E-ISSN 1432-0878, Vol. 332, no 1, 49-56 p.Article in journal (Refereed) Published
National Category
Cardiac and Cardiovascular Systems
Research subject
Cardiology
Identifiers
urn:nbn:se:umu:diva-8347 (URN)10.1007/s00441-007-0562-8 (DOI)18196276 (PubMedID)
Available from: 2008-01-18 Created: 2008-01-18 Last updated: 2010-08-26Bibliographically approved
5. Hyaluronan in human hypertrophic cardiomyopathy: a morphological analysis
Open this publication in new window or tab >>Hyaluronan in human hypertrophic cardiomyopathy: a morphological analysis
Manuscript (Other academic)
Identifiers
urn:nbn:se:umu:diva-2343 (URN)
Available from: 2007-05-10 Created: 2007-05-10 Last updated: 2010-01-13Bibliographically approved

Open Access in DiVA

fulltext(415 kB)716 downloads
File information
File name FULLTEXT01.pdfFile size 415 kBChecksum SHA-1
49317843c04b054d51fd7614fd5537eca0fce5714745e0eea83cb3c19aded9719d414fca
Type fulltextMimetype application/pdf

By organisation
MedicineOtorhinolaryngology

Search outside of DiVA

GoogleGoogle Scholar
Total: 716 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 452 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf