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Low temperature acclimation in plants: alterations in photosynthetic carbon metabolism
Umeå University, Faculty of Science and Technology, Plant Physiology.
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Although low temperature plays an important role in determining agricultural yield, little is known about the effect on the underlying biochemical and physiological processes that influence plant growth. Photosynthesis and respiration are central to plant growth and both processes are heavily affected by temperature. However, many plants have the ability to cope with low temperature and resume growth by cold acclimating.

We have shown that enhancement of carbon fixation, an increased flux of carbon into sucrose and the recovery of diurnal export is crucial for the recovery of functional carbon metabolism at low temperature in Arabidopsis thaliana. The recovery of efflux is governed by increased expression of sucrose transporters along with changes in vascularisation. We also demonstrate the importance of controlling the flux of metabolites between the chloroplast and the cytosol by regulating the expression of AtTPT.

We further investigated the difference in response between leaves developed at low temperature but originating from warm grown Arabidopsis and leaves from plants grown from seed at low temperature. We were able to distinguish factors that respond specifically to low temperature from those that are connected to the actual stress. Substantial difference could be seen in the different metabolomes. One conclusion drawn is that the increase in sucrose reported at low temperature is an essential feature for life in the cold.

In an extended study we were able to transfer some of the key factor of cold acclimation in Arabidopsis to other species. The study included forbs, grasses and evergreen trees/shrubs showed that there are striking similarities in the extent and biochemical changes that underpin acclimation among the different functional groups.

Low temperature does not only influence growth of the leaves, perennial organs such as the corm of the ornamental plant Crocus vernus is also affected. However in these plants low temperature has a positive effect on the final size of the corm. We were able to show that this enhanced growth was an affect of increased cell size and thus increased sink capacity, which ultimately delays leaf senescence

Place, publisher, year, edition, pages
Umeå: Fysiologisk botanik , 2007. , 69 p.
Keyword [en]
Plant physiology, cold acclimation, carbon metabolism, photosynthesis, respiration
Keyword [sv]
Växtfysiologi
Research subject
Physiological Botany
Identifiers
URN: urn:nbn:se:umu:diva-1333ISBN: 978-91-7264-374-1 (print)OAI: oai:DiVA.org:umu-1333DiVA: diva2:140683
Public defence
2007-09-28, KB3B1, KBC huset, Umeå Universitet, Umeå, 10:00
Opponent
Supervisors
Available from: 2007-09-05 Created: 2007-09-05Bibliographically approved
List of papers
1. Carbon partitioning and export in transgenic Arabidopsis thaliana with altered capacity for sucrose synthesisgrown at low temperture: a role for metabolite transporters
Open this publication in new window or tab >>Carbon partitioning and export in transgenic Arabidopsis thaliana with altered capacity for sucrose synthesisgrown at low temperture: a role for metabolite transporters
2006 (English)In: Plant, Cell and Environment, ISSN 0140-7791, E-ISSN 1365-3040, Vol. 29, no 9, 1703-1714 p.Article in journal (Refereed) Published
Abstract [en]

We investigated the role of metabolite transporters in cold acclimation by comparing the responses of wild-type (WT) Arabidopsis thaliana(Heynh.) with that of transgenic plants over-expressing sucrose-phosphate synthase (SPSox) or with that of antisense repression of cytosolic fructose-1,6-bisphosphatase (FBPas). Plants were grown at 23 °C and then shifted to 5 °C. We compared the leaves shifted to 5 °C for 3 and 10 d with new leaves that developed at 5 °C with control leaves on plants at 23 °C. At 23 °C, ectopic expression of SPS resulted in 30% more carbon being fixed per day and an increase in sucrose export from source leaves. This increase in fixation and export was supported by increased expression of the plastidic triose-phosphate transporter AtTPT and, to a lesser extent, the high-affinity Suc transporter AtSUC1. The improved photosynthetic performance of the SPSox plants was maintained after they were shifted to 5 °C and this was associated with further increases in AtSUC1 expression but with a strong repression of AtTPT mRNA abundance. Similar responses were shown by WT plants during acclimation to low temperature and this response was attenuated in the low sucrose producing FBPas plants. These data suggest that a key element in recovering flux through carbohydrate metabolism in the cold is to control the partitioning of metabolites between the chloroplast and the cytosol, and Arabidopsis modulates the expression of AtTPT to maintain balanced carbon flow. Arabidopsis also up-regulates the expression of AtSUC1, and to lesser extent AtSUC2, as down-stream components facilitate sucrose transport in leaves that develop at low temperatures.

Place, publisher, year, edition, pages
Oxford: Blackwell Scientific Publications Ltd, 2006
Keyword
carbon flux, cold acclimation, frost tolerance, photosynthesis
Identifiers
urn:nbn:se:umu:diva-2515 (URN)10.1111/j.1365-3040.2006.01543.x (DOI)16913860 (PubMedID)
Available from: 2007-09-05 Created: 2007-09-05 Last updated: 2017-12-14Bibliographically approved
2. The interaction between development and stress in the low temperature metabolome of Arabidopsis thaliana
Open this publication in new window or tab >>The interaction between development and stress in the low temperature metabolome of Arabidopsis thaliana
Show others...
(English)Manuscript (Other (popular science, discussion, etc.))
Identifiers
urn:nbn:se:umu:diva-2516 (URN)
Available from: 2007-09-05 Created: 2007-09-05 Last updated: 2015-04-29Bibliographically approved
3. Acclimation of photosynthesis and respiration in response to change in growth temperature is asynchronous across plant functional groups
Open this publication in new window or tab >>Acclimation of photosynthesis and respiration in response to change in growth temperature is asynchronous across plant functional groups
Show others...
2007 (English)In: New Phytologist, ISSN 0028-646XArticle in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-2517 (URN)
Available from: 2007-09-05 Created: 2007-09-05 Last updated: 2015-04-29Bibliographically approved
4. Effect of temperature on carbon partitioning, translocation, growth and senescence in the spring ephemeral Crocus vernus
Open this publication in new window or tab >>Effect of temperature on carbon partitioning, translocation, growth and senescence in the spring ephemeral Crocus vernus
Manuscript (Other academic)
Identifiers
urn:nbn:se:umu:diva-2518 (URN)
Available from: 2007-09-05 Created: 2007-09-05 Last updated: 2010-01-13Bibliographically approved

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