Rapid and sensitive method for the analysis of carbon monoxide in blood using gas chromatography with flame ionisation detection
2002 (English)In: Journal of chromatography. B, ISSN 1570-0232, E-ISSN 1873-376X, Vol. 766, no 1, 115-121 p.Article in journal (Refereed) Published
In order to measure changes in physiological CO concentrations in blood with good accuracy, a method was developed using gas chromatography with flame ionisation detection (250 degrees C). A nickel catalyst system was fitted to convert CO to methane at 375 degrees C after separation with a molecular sieve column at 35 degrees C. Helium was used as carrier at 30 ml/min. Porcine or human blood (400 microl) was sampled in gastight tubes and treated with sulfuric acid and saponin (800 microl). Accuracy was 1.4% and 1.5% (RSD), respectively. Precision was 2.8% (porcine blood). Limit of detection was 0.01 nmol/ml gas and limit of quantification 12 nmol/ml blood. Calibration was made in the interval 12-514 nmol/ml blood (corresponding to 0.1-6% COHb). Samples were stable for at least a month at +4 degrees C. This paper describes a method with high sensitivity and good accuracy, suitable for analysis of low CO concentrations.
Place, publisher, year, edition, pages
2002. Vol. 766, no 1, 115-121 p.
Cancer and Oncology
IdentifiersURN: urn:nbn:se:umu:diva-2753DOI: 10.1016/S0378-4347(01)00460-1PubMedID: 11820286OAI: oai:DiVA.org:umu-2753DiVA: diva2:141014