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Tentacle-type Zwitterionic stationary phase, prepared by surface-initiated graft polymerization of 3-[N,N-dimethyl-N-(methacryloyloxyethyl)-ammonium] propanesulfonate through peroxide groups tethered on porous silica
Umeå University, Faculty of Science and Technology, Chemistry.
2002 In: Analytical Chemistry, Vol. 74, no 18, 4682-4687 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
2002. Vol. 74, no 18, 4682-4687 p.
Identifiers
URN: urn:nbn:se:umu:diva-2938OAI: oai:DiVA.org:umu-2938DiVA: diva2:141309
Available from: 2003-11-14 Created: 2003-11-14Bibliographically approved
In thesis
1. Zwitterionic Separation Materials for Liquid Chromatography and Capillary Electrophoresis: Synthesis, Characterization and Application for Inorganic Ion and Biomolecule Separations
Open this publication in new window or tab >>Zwitterionic Separation Materials for Liquid Chromatography and Capillary Electrophoresis: Synthesis, Characterization and Application for Inorganic Ion and Biomolecule Separations
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Liquid Chromatography (LC) and Capillary Electrophoresis (CE) are modern analytical techniques that play very important roles in many areas of modern science such as life science, biotechnology, biomedicine, environmental studies, and development of pharmaceutics. Even though these two techniques have existed and been subjected to studies for several decades, the developments of new separation materials for them are still very important till now in order to meet the different new demands for improvement from other disciplines in science.

In this doctoral thesis, several novel covalently bonded sulfobetaine type zwitterionic separation materials are synthesized for the application in LC and CE. These materials carry both positively charged quaternary ammonium groups and negatively charged sulfonic groups, which result in a very low net surface charge compared to conventional separation materials with only anionic or cationic functional groups. Consequently, it is possible to employ these materials for separation of different ionic species under mild conditions. The surface properties have also been characterized, mainly by elemental analysis, sorption isotherm, ζ-potential measurements, and spectroscopic methods.

By using packed zwitterionic columns for liquid chromatography, small inorganic anions or cations, and acidic or basic proteins can be independently and simultaneously separated in a single run using optimal sets of separation conditions. This is a unique property compared to conventional ionic separation material for LC. When fused silica capillaries coated with zwitterionic polymer are used for capillary electrophoresis, good separations can be achieved for solutes as different as inorganic anions, peptides, proteins, and tryptically digested proteins.

Publisher
53 p.
Keyword
Analytical chemistry, Zwitterionic, stationary phase, separation, liquid chromatography, capillary electrophoresis, sulfobetaine, modification, covalently bonded, graft polymerization, ζ-potential, Analytisk kemi
National Category
Analytical Chemistry
Identifiers
urn:nbn:se:umu:diva-153 (URN)91-7305-543-3 (ISBN)
Public defence
2003-12-05, Umeå, 10:00
Available from: 2003-11-14 Created: 2003-11-14Bibliographically approved

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