umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Zwitterionic Separation Materials for Liquid Chromatography and Capillary Electrophoresis: Synthesis, Characterization and Application for Inorganic Ion and Biomolecule Separations
Umeå University, Faculty of Science and Technology, Chemistry.
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Liquid Chromatography (LC) and Capillary Electrophoresis (CE) are modern analytical techniques that play very important roles in many areas of modern science such as life science, biotechnology, biomedicine, environmental studies, and development of pharmaceutics. Even though these two techniques have existed and been subjected to studies for several decades, the developments of new separation materials for them are still very important till now in order to meet the different new demands for improvement from other disciplines in science.

In this doctoral thesis, several novel covalently bonded sulfobetaine type zwitterionic separation materials are synthesized for the application in LC and CE. These materials carry both positively charged quaternary ammonium groups and negatively charged sulfonic groups, which result in a very low net surface charge compared to conventional separation materials with only anionic or cationic functional groups. Consequently, it is possible to employ these materials for separation of different ionic species under mild conditions. The surface properties have also been characterized, mainly by elemental analysis, sorption isotherm, ζ-potential measurements, and spectroscopic methods.

By using packed zwitterionic columns for liquid chromatography, small inorganic anions or cations, and acidic or basic proteins can be independently and simultaneously separated in a single run using optimal sets of separation conditions. This is a unique property compared to conventional ionic separation material for LC. When fused silica capillaries coated with zwitterionic polymer are used for capillary electrophoresis, good separations can be achieved for solutes as different as inorganic anions, peptides, proteins, and tryptically digested proteins.

Place, publisher, year, edition, pages
2003. , 53 p.
Keyword [en]
Analytical chemistry, Zwitterionic, stationary phase, separation, liquid chromatography, capillary electrophoresis, sulfobetaine, modification, covalently bonded, graft polymerization, ζ-potential
Keyword [sv]
Analytisk kemi
National Category
Analytical Chemistry
Identifiers
URN: urn:nbn:se:umu:diva-153ISBN: 91-7305-543-3 (print)OAI: oai:DiVA.org:umu-153DiVA: diva2:141313
Public defence
2003-12-05, Umeå, 10:00
Available from: 2003-11-14 Created: 2003-11-14Bibliographically approved
List of papers
1. Covalently bonded polymeric zwitterionic stationary phase for simultaneous separation of inorganic cations and anions
Open this publication in new window or tab >>Covalently bonded polymeric zwitterionic stationary phase for simultaneous separation of inorganic cations and anions
1999 In: Analytical Chemistry, Vol. 71, no 2, 333-344 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-2936 (URN)
Available from: 2003-11-14 Created: 2003-11-14Bibliographically approved
2. Synthesis and Evaluation of Polymer-Based Zwitterionic Stationary Phases for Separation of Ionic Species
Open this publication in new window or tab >>Synthesis and Evaluation of Polymer-Based Zwitterionic Stationary Phases for Separation of Ionic Species
2001 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 73, no 9, 1993-2003 p.Article in journal (Refereed) Published
Abstract [en]

Three different zwitterionic functional stationary phases for chromatography were synthesized on the basis of 2-hydroxyethyl methacrylate (HEMA) polymeric particles. Two synthesis routes, producing materials designated S300-ECH-DMA-PS or S300-TC-DMA-PS, involved activation of the hydroxyl groups of the HEMA material with epichlorohydrin or thionyl chloride, respectively, followed by dimethylamination and quaternizing 3-sulfopropylation with 1,3-propane sultone. The third route was accomplished by attaching methacrylate moieties to the HEMA through a reaction with methacrylic anhydride, followed by graft photopolymerization of the zwitterionic monomer 3-[N,N-dimethyl-N-(methacryloyloxyethyl)ammonium] propanesulfonate, initiated by benzoin methyl ether under 365-nm light. According to elemental analyses, both the S300-ECH-DMA-PS and S300-TC-DMA-PS materials appeared to have overall charge stoichometries close to unity, whereas the grafted material, S300-MAA-SPE, seemed to carry an excess of anion exchange sites in addition to the zwitterionic groups. Yet all three zwitterionic stationary phases were capable of separating inorganic anions and cations simultaneously and independently using aqueous solutions of perchloric acid or perchlorate salts as eluent, albeit with markedly different selectivities. On the S300-TC-DMA-PS and S300-MAA-SPE materials, the retention times increased for cations and decreased for anions with increasing eluent concentration, whereas with the S300-ECH-DMA-PS material, the retention times of both anions and cations decreased with increasing eluent concentration. These results demonstrate the importance of choosing appropriate synthesis conditions in order to prepare covalently bonded zwitterionic separation materials with an acceptable charge balance.

Place, publisher, year, edition, pages
American Chemical Society (ACS), 2001
National Category
Chemical Sciences
Identifiers
urn:nbn:se:umu:diva-2937 (URN)10.1021/ac000933d (DOI)
Available from: 2003-11-14 Created: 2003-11-14 Last updated: 2017-12-14Bibliographically approved
3. Tentacle-type Zwitterionic stationary phase, prepared by surface-initiated graft polymerization of 3-[N,N-dimethyl-N-(methacryloyloxyethyl)-ammonium] propanesulfonate through peroxide groups tethered on porous silica
Open this publication in new window or tab >>Tentacle-type Zwitterionic stationary phase, prepared by surface-initiated graft polymerization of 3-[N,N-dimethyl-N-(methacryloyloxyethyl)-ammonium] propanesulfonate through peroxide groups tethered on porous silica
2002 In: Analytical Chemistry, Vol. 74, no 18, 4682-4687 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-2938 (URN)
Available from: 2003-11-14 Created: 2003-11-14Bibliographically approved
4. Characterization of polymeric zwitterionic stationary phase and application for protein separation
Open this publication in new window or tab >>Characterization of polymeric zwitterionic stationary phase and application for protein separation
(English)Manuscript (preprint) (Other academic)
National Category
Chemical Sciences
Identifiers
urn:nbn:se:umu:diva-2939 (URN)
Available from: 2003-11-14 Created: 2003-11-14 Last updated: 2017-05-22Bibliographically approved
5. Control of electroosmotic flow and wall interactions in capillary electrophosesis capillaries by photografted zwitterionic polymer surface layers
Open this publication in new window or tab >>Control of electroosmotic flow and wall interactions in capillary electrophosesis capillaries by photografted zwitterionic polymer surface layers
2003 In: Analytical Chemistry, Vol. 75, no 11, 2768-2774 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-2940 (URN)
Available from: 2003-11-14 Created: 2003-11-14Bibliographically approved
6. New Way to Synthesize Zwitterionic Capillary and Its Applications for Determination of Inorganic Anions and Peptides
Open this publication in new window or tab >>New Way to Synthesize Zwitterionic Capillary and Its Applications for Determination of Inorganic Anions and Peptides
Manuscript (Other academic)
Identifiers
urn:nbn:se:umu:diva-2941 (URN)
Available from: 2003-11-14 Created: 2003-11-14 Last updated: 2010-01-13Bibliographically approved

Open Access in DiVA

fulltext(1104 kB)5704 downloads
File information
File name FULLTEXT01.pdfFile size 1104 kBChecksum SHA-1
5bbbf8dbea59fe61aaa84272803a8c40d4e535295ceb4c4f0afd8471acd60ef7e7c1ef36
Type fulltextMimetype application/pdf

By organisation
Chemistry
Analytical Chemistry

Search outside of DiVA

GoogleGoogle Scholar
Total: 5704 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 1364 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf