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Regulation of a novel Acidithiobacillus caldus gene cluster involved in metabolism of reduced inorganic sulfur compounds
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). (Lindström)
Center for Bioinformatics and Genome Biology, Life Science Foundation, MIFAB and Andrés Bello University, Santiago, Chile.
Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Mokslininku 12, Vilnius LT-08662, Lithuania.
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology).
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2007 (English)In: Applied and Environmental Microbiology, ISSN 0099-2240, E-ISSN 1098-5336, Vol. 73, no 22, 7367-7372 p.Article in journal (Refereed) Published
Abstract [en]

Acidithiobacillus caldus has been proposed to play a role in the oxidation of reduced inorganic sulfur compounds (RISCs) produced in industrial biomining of sulfidic minerals. Here, we describe the regulation of a new cluster containing the gene encoding tetrathionate hydrolase (tetH), a key enzyme in the RISC metabolism of this bacterium. The cluster contains five cotranscribed genes, ISac1, rsrR, rsrS, tetH, and doxD, coding for a transposase, a two-component response regulator (RsrR and RsrS), tetrathionate hydrolase, and DoxD, respectively. As shown by quantitative PCR, rsrR, tetH, and doxD are upregulated to different degrees in the presence of tetrathionate. Western blot analysis also indicates upregulation of TetH in the presence of tetrathionate, thiosulfate, and pyrite. The tetH cluster is predicted to have two promoters, both of which are functional in Escherichia coli and one of which was mapped by primer extension. A pyrrolo-quinoline quinone binding domain in TetH was predicted by bioinformatic analysis, and the presence of an o-quinone moiety was experimentally verified, suggesting a mechanism for tetrathionate oxidation.

Place, publisher, year, edition, pages
2007. Vol. 73, no 22, 7367-7372 p.
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-2961DOI: 10.1128/AEM.01497-07PubMedID: 17873067OAI: oai:DiVA.org:umu-2961DiVA: diva2:141348
Available from: 2008-02-15 Created: 2008-02-15 Last updated: 2013-02-15Bibliographically approved
In thesis
1. Physiology and Genetics of Acidithiobacillus species: Applications for Biomining
Open this publication in new window or tab >>Physiology and Genetics of Acidithiobacillus species: Applications for Biomining
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bacteria from the genus Acidithiobacillus are often associated with biominingand acid mine drainage. Biomining utilises acidophilic, sulphur and ironoxidising microorganisms for recovery of metals from sulphidic low grade oresand concentrates. Acid mine drainage results in acidification and contaminationwith metals of soil and water emanating from the dissolution of metal sulphidesfrom deposits and mine waste storage. Acidophilic microorganisms play acentral role in these processes by catalysing aerobic oxidation of sulphides.Acceleration of mineral solubilisation is a positive aspect in biomining whereas,in acid mine drainage it is undesirable and accordingly, microbial iron andsulphur oxidation is promoted in the first case and measures are taken to inhibitit in the second case. In this thesis, several approaches were taken in order tounderstand and increase oxidation efficiency in biomining and to gain an insightinto the biochemical reactions taking place in these environments. A laboratoryscale bioreactor was designed and tested allowing simulation of bioleaching inheaps of mine tailings at different aeration, irrigation and particle size conditions(Paper I). A new psychrotolerant strain of Acidithiobacillus ferrooxidans wascharacterised that has an application in boreal heap bioleaching. Iron, reducedinorganic sulphur compound oxidation and bioleaching of various ores by thisstrain was studied as well as gene expression during oxidation of tetrathionateand/or ferrous iron (Papers III & IV). Expression and regulation of atetrathionate hydrolase from Acidithiobacillus caldus, a key enzyme in reducedinorganic sulphur compound metabolism of this bacterium was investigated andthe presence of this enzyme in a bioleaching mixed culture was shown. The genecluster that harbours the gene coding for tetrathionate hydrolase (tetH) wasdescribed for the first time (Paper II).

Place, publisher, year, edition, pages
Umeå: Molekylärbiologi (Teknisk-naturvetenskaplig fakultet), 2008. 73 p.
National Category
Microbiology
Identifiers
urn:nbn:se:umu:diva-1545 (URN)978-91-7264-509-7 (ISBN)
Public defence
2008-03-07, Major Grove, 6L, Umeå University, Dept. of Molecular Biology, 10:00 (English)
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Available from: 2008-02-15 Created: 2008-02-15 Last updated: 2009-06-30Bibliographically approved

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Publisher's full textPubMedhttp://dx.doi.org/10.1128/AEM.01497-07

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Rzhepishevska, Olena IDopson, Mark

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