umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Planar lipid bilayers on solid supports from liposomes: factors of importance for kinetics and stability
Umeå University, Faculty of Science and Technology, Chemistry.
1997 In: Biochimica et Biophysica Acta, Vol. 1327, 149-161 p.Article in journal (Refereed) Published
Place, publisher, year, edition, pages
1997. Vol. 1327, 149-161 p.
Identifiers
URN: urn:nbn:se:umu:diva-3303OAI: oai:DiVA.org:umu-3303DiVA: diva2:141878
Available from: 2003-12-22 Created: 2003-12-22Bibliographically approved
In thesis
1. Phospholipid membranes in biosensor applications: Stability, activity and kinetics of reconstituted proteins and glycolipids in supported membranes
Open this publication in new window or tab >>Phospholipid membranes in biosensor applications: Stability, activity and kinetics of reconstituted proteins and glycolipids in supported membranes
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this study the formation of supported membranes onto planar solid supports has been investigated. The stability and activity of reconstituted membrane receptors has been studied. The potential use of such preparations in biosensor applications is discussed.

The lipid films were made by the Langmuir Blodgett and by the liposome fusion techniques. These supported films were characterised by ellipsometry, atomic force microscopy, surface plasmon resonance (SPR) and resonant mirror techniques. The thickness of the films was in agreement with that of a cell membrane. The kinetics of formation of the lipid films was studied and discussed.

The proteins, bacteriorhodopsin, cytochrome oxidase, acetylcholinesterase and the nicotinic acetylcholine receptor were reconstituted into the supported membrane. The subsequent analysis showed that the proteins were individually distributed and that the activity was retained, in some cases for several weeks after immobilisation.

The glycolipids, GM1, GM2, GD1b, asialo-GM1, globotriaosylceramide, lactosylceramide and galactosylceramide, were also reconstituted into the supported membranes. Their specific interaction with the toxin ricin or with its B-chain was examined using SPR. The affinity of intact toxin and of its B-chain differed markedly and was pH dependent. The carbohydrate chain length and charge density of the glycolipids also influenced the affinity.

Publisher
123 p.
Series
FOI-R, ISSN 1650-1942 ; 987
Keyword
Biochemistry, Phospholipid films, Liposomes, Membrane proteins, Glycolipids, Ellipsometry, Langmuir Blodgett (LB), Surface plasmon resonance (SPR), Resonant mirror (RM), Atomic force microscopy (AFM), Biosensors, Biokemi
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
urn:nbn:se:umu:diva-172 (URN)91-7305-547-6 (ISBN)
Public defence
2004-01-16, A9, KB3, KB-huset, Umeå, 10:00
Opponent
Supervisors
Available from: 2003-12-22 Created: 2003-12-22Bibliographically approved

Open Access in DiVA

No full text

Other links

http://dx.doi.org/10.1016/S0005-2736(97)00052-7
By organisation
Chemistry

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 23 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf