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Phospholipid membranes in biosensor applications: Stability, activity and kinetics of reconstituted proteins and glycolipids in supported membranes
Umeå University, Faculty of Science and Technology, Chemistry.
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In this study the formation of supported membranes onto planar solid supports has been investigated. The stability and activity of reconstituted membrane receptors has been studied. The potential use of such preparations in biosensor applications is discussed.

The lipid films were made by the Langmuir Blodgett and by the liposome fusion techniques. These supported films were characterised by ellipsometry, atomic force microscopy, surface plasmon resonance (SPR) and resonant mirror techniques. The thickness of the films was in agreement with that of a cell membrane. The kinetics of formation of the lipid films was studied and discussed.

The proteins, bacteriorhodopsin, cytochrome oxidase, acetylcholinesterase and the nicotinic acetylcholine receptor were reconstituted into the supported membrane. The subsequent analysis showed that the proteins were individually distributed and that the activity was retained, in some cases for several weeks after immobilisation.

The glycolipids, GM1, GM2, GD1b, asialo-GM1, globotriaosylceramide, lactosylceramide and galactosylceramide, were also reconstituted into the supported membranes. Their specific interaction with the toxin ricin or with its B-chain was examined using SPR. The affinity of intact toxin and of its B-chain differed markedly and was pH dependent. The carbohydrate chain length and charge density of the glycolipids also influenced the affinity.

Place, publisher, year, edition, pages
2004. , 123 p.
Series
FOI-R, ISSN 1650-1942 ; 987
Keyword [en]
Biochemistry, Phospholipid films, Liposomes, Membrane proteins, Glycolipids, Ellipsometry, Langmuir Blodgett (LB), Surface plasmon resonance (SPR), Resonant mirror (RM), Atomic force microscopy (AFM), Biosensors
Keyword [sv]
Biokemi
National Category
Biochemistry and Molecular Biology
Research subject
Biochemistry
Identifiers
URN: urn:nbn:se:umu:diva-172ISBN: 91-7305-547-6 (print)OAI: oai:DiVA.org:umu-172DiVA: diva2:141881
Public defence
2004-01-16, A9, KB3, KB-huset, Umeå, 10:00
Opponent
Supervisors
Available from: 2003-12-22 Created: 2003-12-22Bibliographically approved
List of papers
1. Retained activities of some membrane proteins in stable lipid bilayer on a solid support
Open this publication in new window or tab >>Retained activities of some membrane proteins in stable lipid bilayer on a solid support
1995 In: Biosensors & Bioelectronics, Vol. 10, 463-476 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-3302 (URN)
Available from: 2003-12-22 Created: 2003-12-22Bibliographically approved
2. Planar lipid bilayers on solid supports from liposomes: factors of importance for kinetics and stability
Open this publication in new window or tab >>Planar lipid bilayers on solid supports from liposomes: factors of importance for kinetics and stability
1997 In: Biochimica et Biophysica Acta, Vol. 1327, 149-161 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-3303 (URN)
Available from: 2003-12-22 Created: 2003-12-22Bibliographically approved
3. Distribution and stability of membrane proteins in lipid membranes on solid supports
Open this publication in new window or tab >>Distribution and stability of membrane proteins in lipid membranes on solid supports
Show others...
2000 In: Biosensors & Bioelectronics, Vol. 15, 31-41 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-3304 (URN)
Available from: 2003-12-22 Created: 2003-12-22Bibliographically approved
4. Investigating the interaction of the toxin ricin and its B-chain with immobilised glycolipids in supported phospholipid membranes by surface plasmon resonance
Open this publication in new window or tab >>Investigating the interaction of the toxin ricin and its B-chain with immobilised glycolipids in supported phospholipid membranes by surface plasmon resonance
2003 In: Colloids and Surfaces B: Biointerfaces, Vol. 30, 13-24 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-3305 (URN)
Available from: 2003-12-22 Created: 2003-12-22Bibliographically approved

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Citation style
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