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Type IV pili are not specifically required for contact dependent translocation of exoenzymes by Pseudomonas aeruginosa
Umeå University, Faculty of Science and Technology, Department of Molecular Biology (Faculty of Science and Technology). Department of Medical Countermeasures, FOI NBC-Defence, S-901 82 Umeå, Sweden.
Umeå University, Faculty of Medicine, Department of Molecular Biology (Faculty of Medicine). Department of Medical Countermeasures, FOI NBC-Defence, S-901 82 Umeå, Sweden.
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2002 (English)In: Microbial Pathogenesis, ISSN 0882-4010, Vol. 33, no 6, p. 265-277Article in journal (Refereed) Published
Abstract [en]

The type III secretion system (TTSS) of the opportunistic pathogen Pseudomonas aeruginosa enables the bacterium to deliver exoenzymes directly into the eukaryotic cell. In this study we have investigated the role of key factors involved in this process. We could demonstrate that the translocators PopB, PopD and PcrV are absolutely required for delivery of Exoenzyme S into host cells. By analyzing different Tfp (type IV pili) mutants we could establish a correlation between the frequency of bacteria binding to the host cell and the levels of translocated ExoS, thereby verifying that the process is contact dependent. However, there was no absolute requirement for the Tfp per se, since the pilus could be substituted with a different type of adhesin, the non-fimbrial adhesin pH6 antigen of Yersinia pestis. Taken together, our results demonstrate that binding to establish close contact between the type III secretion organelle and the host cell is essential for translocation, while the additional activities of Tfp are not essential for the delivery of TTSS proteins.

Place, publisher, year, edition, pages
Elsevier, 2002. Vol. 33, no 6, p. 265-277
Keywords [en]
Pseudomonas aeruginosa, type III secretion, type IV pili, ExoS, PcrV, PopB, PopD, pH6 antigen, Ras
National Category
Cell and Molecular Biology
Identifiers
URN: urn:nbn:se:umu:diva-3306DOI: 10.1006/mpat.2002.0534ISI: 000180403600002PubMedID: 12495673OAI: oai:DiVA.org:umu-3306DiVA, id: diva2:141902
Available from: 2003-12-23 Created: 2003-12-23 Last updated: 2019-01-24Bibliographically approved
In thesis
1. Type III Secretion Mediated Translocation of Effector Exoenzymes by Pseudomonas aeruginosa
Open this publication in new window or tab >>Type III Secretion Mediated Translocation of Effector Exoenzymes by Pseudomonas aeruginosa
2003 (English)Doctoral thesis, comprehensive summary (Other academic)
Alternative title[sv]
Injektion av gifter via typ III sekretionssystemet hos bakterien Pseudomonas aeruginosa
Publisher
p. 52
Keywords
Cell and molecular biology, Pseudomonas aeruginosa, type III secretion system, ExoS, ExoT, PcrV, PcrG, PopB, PopD, PopN, type IV pili, Cell- och molekylärbiologi
National Category
Biochemistry and Molecular Biology
Research subject
Molecular Biology
Identifiers
urn:nbn:se:umu:diva-174 (URN)91-7305-545-X (ISBN)
Public defence
2003-11-28, Major Groove, 6L, Norrlands Universitetssjukhus, Umeå, 09:00 (English)
Opponent
Supervisors
Available from: 2003-12-23 Created: 2003-12-23 Last updated: 2019-01-24Bibliographically approved

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Sundin, CharlottaForsberg, Åke

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