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Diurnal effects of enriched environment in immediate early gene expression in the rat brain
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine.
Umeå University, Faculty of Medicine, Department of Public Health and Clinical Medicine.
2005 (English)In: Brain research, ISSN 0006-8993, Vol. 1046, no 1-2, 137-144 p.Article in journal (Refereed) Published
Abstract [en]

Rodents housed in an enriched environment (EE) show increased neuronal plasticity with enhanced long-term potentiation and memory performance. We report an EE-induced increase in NGFI-A and Krox-20 mRNA expression exclusively during the dark period of the day. In addition, EE-housed rats showed considerable diurnal variation in NGFI-A, Krox-20, and NGFI-B mRNA expression which was absent in single-housed rats. Thus, EE-induced molecular changes are more evident during the dark phase when the rats have higher motor and exploratory activity. This is important to take into account in future studies of molecular mediators of experience-dependent neuronal plasticity.

Place, publisher, year, edition, pages
2005. Vol. 1046, no 1-2, 137-144 p.
Keyword [en]
analysis of variance, animals, brain/metabolism, circadian rhythm/genetics/*physiology, DNA-binding proteins/genetics/*metabolism, darkness, early growth response protein 1, early growth response protein 2, environment, gene expression/physiology, immediate-early proteins/genetics/*metabolism, male, neuronal plasticity/physiology, RNA, messenger/analysis, rats, sprague-dawley, Receptors, cytoplasmic and nuclear/genetics/metabolism, receptors, steroid/genetics/metabolism, transcription factors/genetics/*metabolism
Identifiers
URN: urn:nbn:se:umu:diva-3999DOI: doi:10.1016/j.brainres.2005.03.051PubMedID: 15927552OAI: oai:DiVA.org:umu-3999DiVA: diva2:142930
Available from: 2004-05-12 Created: 2004-05-12 Last updated: 2010-08-19Bibliographically approved
In thesis
1. The effect of enriched environment on gene expression and stroke recovery
Open this publication in new window or tab >>The effect of enriched environment on gene expression and stroke recovery
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Stroke is the third leading cause of death and the major course of long-term disabilities in industrialized countries. Most surviving stroke patients show some degree of spontaneous recovery, but persistent symptoms in sensorimotor and cognitive functions are common. The symptoms can be reproduced in experimental stroke models in rats by occlusion of the middle cerebral artery. Housing rats in an enriched environment (EE), i.e. group housing in a large cage with toys that are changed daily, increases neuronal plasticity in healthy rats and can also improve functional recovery after experimental stroke.

The present thesis investigates the effect of EE on the recovery of sensorimotor and cognitive functions one month after focal cerebral ischemia in rats, with emphasis on the underlying molecular mechanisms. Furthermore, EE-induced effect on gene expression in healthy rats was investigated after different periods of EE-housing and at different time points of the day.

We show an improved recovery of both sensorimotor and cognitive functions in rats housed in EE for one month after focal cerebral ischemia. The recovery of sensorimotor function correlated significantly to mRNA expression of the plasticity associated transcription factors NGFI-A and NGFI-B in hippocampus and cortical regions outside the infarct. Social interaction seems to be an important component for the beneficial effects of EE after focal cerebral ischemia. Microarray analysis of hippocampal gene expression after one month of postischemic environmental enrichment revealed no confirmable EE-induced changes that could explain the improved recovery in spatial memory. Interestingly, healthy rats housed in EE showed increased mRNA expression of NGFI-A and Krox-20 exclusively during the dark period of the day compared to rats housed in isolation. In addition, EE housed rats had a substantial diurnal variation in NGFI-A, Krox-20 and NGFI-B mRNA expression; this was absent in single-housed rats. EE-induced changes in gene expression are more evident during the dark period of the day, when rats are more active and can benefit from the stimulating environment. This is important to consider in future investigation of putative mediators of the EE-induced neuronal plasticity.

In summary, these findings may contribute to an increased understanding of the underlying molecular mechanisms behind improved functional recovery in rats housed in enriched environment after focal cerebral ischemia.

Publisher
115 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 883
Keyword
Medicine, experimental stroke, recovery, memory, inducible transcription factors, Medicin
National Category
Dermatology and Venereal Diseases
Identifiers
urn:nbn:se:umu:diva-278 (URN)91-7305-626-X (ISBN)
Public defence
2004-06-04, Sal B, plan 9, By 1D, Tandläkarhögskolan, Norrlands universitetssjukhus, Umeå, 13:00
Opponent
Supervisors
Available from: 2004-05-12 Created: 2004-05-12Bibliographically approved

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Rönnbäck, AnnicaDahlqvist, PerBergström, Sven-AndersOlsson, Tommy
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