Interferon-alpha promotes survival of human primary B-lymphocytes via phoshatidylinositol 3-kinase
2001 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, ISSN --, Vol. 284, no 3, 583-586 p.Article in journal (Refereed) Published
Signaling pathways for the antiviral and antiproliferative biological effects of type I interferons (IFN) are well established. In this report we demonstrate a novel signaling pathway for IFN-α, as it induced rapid phosphorylation of both PKB/Akt and its substrate forkhead. The PI3-kinase inhibitor LY294002 abolished these phosphorylations. PI3-kinase has been implicated in cell survival mediating its effect through the second messenger PIP3 and the subsequent activation of PKB/Akt. We could show that IFN-α inhibited spontaneous apoptosis of primary B-lymphocytes, in the absence of a mitogenic stimulus. This effect was inhibited by LY294002. Thus, our data suggests that IFN-α promotes survival of peripheral B-lymphocytes via the PI3-kinase-PKB/Akt pathway. In addition, IFN-α stimulation of anti-IgM activated cells resulted in downregulated expression of the cell cycle inhibitor p27/Kip1.
Place, publisher, year, edition, pages
2001. Vol. 284, no 3, 583-586 p.
apoptosis, B-lymphocytes, interferon, phosphatidylinositol 3-kinase, PKB/Akt; forkhead, p27/Kip1
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-4109DOI: 10.1006/bbrc.2001.5025OAI: oai:DiVA.org:umu-4109DiVA: diva2:143079