Comparative analysis of type III effector translocation by Yersinia pseudotuberculosis expressing native LcrV or PcrV from Pseudomonas aeruginosa.
2003 (English)In: Journal of Infectious Diseases, ISSN 0022-1899, Vol. 188, no 2, 239-249 p.Article in journal (Refereed) Published
The homologues LcrV of Yersinia species and PcrV of Pseudomonas aeruginosa are pore-forming components. When expressed in a Yersinia lcrV background, PcrV formed smaller pores in infected erythrocyte membranes, correlating to a lowered translocation of Yersinia effectors. To understand this phenomenon, cytotoxins exoenzyme S of P. aeruginosa and YopE of Yersinia were introduced into a Yersinia background without Yop effectors but expressing LcrV or PcrV. Comparable translocation of each substrate indicated that substrate recognition by LcrV/PcrV is not a regulator of translocation. Yersinia harboring pcrV coexpressed with its native operon efficiently translocated effectors into HeLa cell monolayers and formed large LcrV-like pores in erythrocyte membranes. Thus, a PcrV complex with native P. aeruginosa translocon components is required to form fully functional pores for complete complementation of effector translocation in Yersinia.
Place, publisher, year, edition, pages
2003. Vol. 188, no 2, 239-249 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-4144DOI: 10.1086/376452PubMedID: 12854079OAI: oai:DiVA.org:umu-4144DiVA: diva2:143125