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Characterization of the tetratricopeptide repeats in type III secretion chaperones- mediators of substrate binding and specificity.
Umeå University, Faculty of Medicine, Molecular Biology.
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Manuscript (Other academic)
URN: urn:nbn:se:umu:diva-4147OAI: diva2:143128
Available from: 2004-10-06 Created: 2004-10-06 Last updated: 2010-01-13Bibliographically approved
In thesis
1. Type III secretion- the various functions of the translocon operon in bacterial pathogenesis
Open this publication in new window or tab >>Type III secretion- the various functions of the translocon operon in bacterial pathogenesis
2004 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In order to establish colonisation of a human host, pathogenic Yersinia use a type III protein secretion system to directly intoxicate host immune cells. Activation of this system requires target cell contact and is a highly regulated process. Both the intoxication and regulation events depend on the lcrGVHyopBD translocon operon, which is highly conserved in many bacterial pathogens. In this study, the role of individual operon members was analysed and functional domains identified by using the highly homologous pcrGVHpopBD operon of P. aeruginosa as a comparative tool.

Yersinia spp. and P. aeruginosa were shown to form translocation pores of a similar size that promoted equally efficient protein delivery. A strong dependency on interactions between native translocator(s) in protein delivery was revealed, suggesting that each pathogen has delicately fine-tuned this process to suit its own infection niche. In particular, the C-terminus of YopD was shown to possess functional specificity for effector delivery in Yersinia that could not be conferred by the comparable region in homologous PopD. Moreover, a role for LcrV and PcrV in substrate recognition during the protein delivery process was excluded.

The N-terminus of LcrH was recognized as a unique regulatory domain, mediating formation of LcrH-YscY regulatory complexes in Yersinia, while equivalent complexes with analogous proteins were not formed in P. aeruginosa. These results compliment the idea that a negative regulatory pathway involving LcrH, YopD, LcrQ and YscY is unique to Yersinia.

Finally, PcrH was identified as a new member of the translocator class of chaperones, being essential for assembly of a functional PopB/PopD mediated translocon in P. aeruginosa. However, in contrast to the other members of this family, PcrH was dispensable for type III regulation. Moreover, both LcrH and PcrH were shown to possess tetratricopeptide repeats crucial for their chaperone function. One tetratricopeptide repeat mutant in LcrH was even isolated that failed to secrete both YopB and YopD substrates, even though stability was maintained. This demonstrates for the first time that LcrH has a role in substrate secretion in addition to its critical role in promoting substrate stability.

Place, publisher, year, edition, pages
Umeå: Molekylärbiologi, 2004. 82 p.
Molecular biology, Yersinia, Pseudomonas aeruginosa, type III secretion, chaperone, translocation, regulation, lcrGVHyopBD, pcrGVHpopBD, Molekylärbiologi
National Category
Biochemistry and Molecular Biology
Research subject
Molecular Biology
urn:nbn:se:umu:diva-331 (URN)91-7305-712-6 (ISBN)
Public defence
2004-11-05, Major Groove, 6L, NUS, Umeå Universitet, 90187, Umeå, 09:00
Available from: 2004-10-06 Created: 2004-10-06Bibliographically approved

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