133Xe clearance estimates the effect of vasopressin on peritoneal blood flow in rats
2004 (English)In: Hepato-Gastroenterology, ISSN 0172-6390, Vol. 51, no 58, 1037-1041 p.Article in journal (Refereed) Published
BACKGROUND/AIMS: When assessing the peritoneal microcirculation with invasive methods, interference with the mechanisms of vaso-regulation may occur. The 133Xe clearance technique renders the possibility, by minimal invasiveness, to estimate the influence of a vasoactive agent on the peritoneal microcirculation. METHODOLOGY: Ten to 15MBq of 133Xe were injected in the abdominal cavity in thirty-eight Wistar-FU (W-FU) rats and 35 Lister-Hooded (LH) rats. A NaI (Tl)-scintillation detector registered activity before and during vasopressin infusion. Gamma camera imaging confirmed the washout from the abdominal cavity. The laser Doppler flowmetry technique was used as a comparison. RESULTS: Vasopressin at 0.07 IU/kg/min IV significantly reduced 133Xe-clearance by 37% (p = 0.029) and 52% (p=0.036) and laser Doppler flowmetry by 69% (p=0.0019) and 44% (p=0.0039) in W-FU and LH rats, respectively. A linear correlation between dose of vasopressin and relative decrease in 133Xe clearance was demonstrated in the W-FU rat model (r2=0.98, p=0.023). The 133Xe clearance from the abdominal cavity in rat using a single-compartment model or the slow compartment in a double-compartment model gave reproducible information. CONCLUSIONS: The usefulness of this mini-invasive technique for sequential measurements before and during intervention will render the 133Xe clearance suitable for assessment of blood flow changes in the abdominal cavity.
Place, publisher, year, edition, pages
2004. Vol. 51, no 58, 1037-1041 p.
Animals, Blood Pressure/drug effects, Gamma Cameras, Laser-Doppler Flowmetry, Microcirculation/drug effects, Peritoneum/*blood supply/radiography, Rats, Rats; Inbred Strains, Rats; Wistar, Regional Blood Flow/drug effects, Vasoconstrictor Agents/*pharmacology, Vasopressins/*pharmacology, Xenon Radioisotopes/*pharmacokinetics
IdentifiersURN: urn:nbn:se:umu:diva-4271PubMedID: 15239241OAI: oai:DiVA.org:umu-4271DiVA: diva2:143289