The basic helix-loop-helix transcription factor E2-2 is involved in T lymphocyte development
2000 (English)In: European Journal of Immunology, ISSN 0014-2980, Vol. 30, no 10, 2857-2863 p.Article in journal (Refereed) Published
E2A, HEB and E2-2 genes encode a group of basic helix-loop-helix (bHLH) transcription factors that are structurally and functionally similar. Deletion of the genes encoding either of these proteins leads to early lethality and a block in B lymphocyte development. Evidence for a function in T lymphocyte development has, however, only been reported for E2A and HEB. To further elucidate the role of E2-2 at developmental stages that have proven difficult to study due to the early lethality phenotype of mice defective in E2-2, we generated and analyzed mice conditionally mutated in the E2-2 gene. These mice are mosaic with respect to E2-2 expression, consisting of cells with either one functional and one null mutated E2-2 allele or two null mutated alleles. Using this experimental model, we find that cells with a homozygous null mutated E2-2 gene are under-represented in B lymphocyte as well as T lymphocyte cell lineages as compared to other hematopoietic or non-hematopoietic cell lineages. Our data suggests that E2-2 deficiency leads to a partial block in both B and T lymphocyte development. The block in T cell development appears to occur at an early stage in differentiation, since skewing in the mosaicism is observed already in CD4+8+ double-positive thymocytes.
Place, publisher, year, edition, pages
2000. Vol. 30, no 10, 2857-2863 p.
IdentifiersURN: urn:nbn:se:umu:diva-4592DOI: 10.1002/1521-4141(200010)30:10<2857::AID-IMMU2857>3.0.CO;2-GOAI: oai:DiVA.org:umu-4592DiVA: diva2:143751