umu.sePublications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Hyaluronan in bone marrow in patients with various malignant disorders
Umeå University, Faculty of Medicine, Public Health and Clinical Medicine.
Show others and affiliations
Manuscript (Other academic)
Identifiers
URN: urn:nbn:se:umu:diva-4876OAI: oai:DiVA.org:umu-4876DiVA: diva2:144143
Available from: 2005-12-15 Created: 2005-12-15 Last updated: 2010-01-13Bibliographically approved
In thesis
1. Hyaluronan in normal and malignant bone marrow: a clinical and morphological study with emphasis on myelofibrosis
Open this publication in new window or tab >>Hyaluronan in normal and malignant bone marrow: a clinical and morphological study with emphasis on myelofibrosis
2005 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Fibrosis in the bone marrow is usually denominated myelofibrosis and may contribute to impaired hematopoiesis. Myelofibrosis is seen both in malignant and non-malignant diseases.

The normal microenvironment in the bone marrow consists of a heterogenous population of hematopoietic and non-hematopoietic stromal cells, their extracellular products and hematopoietic cytokines. The stromal cells produce a complex array of molecules, among others collagens and glycosaminoglycans (GAGs) of which hyaluronan (HYA) is the most abundant. Marrow fibrosis results from an increased deposition of collagens, which are polypeptides. Staining for reticulin, mostly composed of collagen type III, is the common way of visualizing myelofibrosis. HYA, like the collagens, is widely distributed in connective tissues. Little is known about the distribution of HYA in bone marrow.

The aims of this thesis have been to determine how HYA is distributed in normal and malignant bone marrow, compared to reticulin staining, and to follow patients with chronic myeloproliferative diseases (CMPD) during two years treatment with anagrelide considering development of cellularity and fibrosis.

In bone marrow biopsies from healthy volunteers, the controls, HYA was found in a pattern that was concordant with the reticulin staining.

Comparing patients with different malignant diseases with and without bone marrow involvemen, HYA staining was found to be significantly stronger in both groups compared to the controls.

The HYA scores were also significantly higher in the bone marrow of patients with de novo acute myeloid leukemia (AML), compared to the controls.

There was a correlation between HYA and reticulin in the patients with de novo AML, and in the patients with different malignant diseases with and without bone marrow involvement as in the controls.

Increase of HYA, reticulin and cellularity in the bone marrow of patients with CMPD after two years of treatment with anagrelide indicated progression of fibrosis. Anagrelide is a valuable drug for reduction of platelets but seems unable to stop progression of fibrosis and hypercellularity.

HYA is an interesting molecule with properties not only contributing to the structure of extracellular matrix but also to cell signaling and behaviour, although the understanding of the detailed mechanisms is still incomplete.

Place, publisher, year, edition, pages
Umeå: Folkhälsa och klinisk medicin, 2005. 60 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 997
Keyword
Hyaluronan, reticulin, fibrosis, chronic myeloproliferative disorders, acute myeloid leukemia, anagrelide, bone marrow
National Category
Clinical Science
Identifiers
urn:nbn:se:umu:diva-658 (URN)91-7305-990-0 (ISBN)
Public defence
2006-02-03, Sal D, Tandläkarhögskolan, Norrlands Universitetssjukhus, Umeå, 13:00 (English)
Opponent
Supervisors
Available from: 2005-12-15 Created: 2005-12-15 Last updated: 2009-10-08Bibliographically approved

Open Access in DiVA

No full text

By organisation
Public Health and Clinical Medicine

Search outside of DiVA

GoogleGoogle Scholar

urn-nbn

Altmetric score

urn-nbn
Total: 18 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf