Cell-specific Ca2+ responses in glucose-stimulated single and aggregated ß-cells
2003 (English)In: Cell Calcium, ISSN 0143-4160, E-ISSN 1532-1991, Vol. 34, no 2, 121-129 p.Article in journal (Refereed) Published
A rise in the cytoplasmic calcium concentration ([Ca(2+)](i)) is a key event for insulin exocytosis. We have recently found that the 'early [Ca(2+)](i) response' in single ob/ob mouse beta-cells is reproduced during consecutive glucose stimulations. It, therefore, appears that the response pattern is a characteristic of the individual beta-cell. We have now investigated if a cell-specific [Ca(2+)](i) response is a general phenomenon in rodent beta-cells, and if it can be observed when cells are functionally coupled. With the use of the fura-2 technique, we have studied the 'early [Ca(2+)](i) response' in single dispersed beta-cells, in beta-cell clusters of different size and in intact islets from the ob/ob mouse during repeated glucose stimulation (20mM). beta-Cells from lean mouse and rat, and intact islets from lean mouse were also investigated. Significant correlations between the first and second stimulation were found for the parameters lag-time for Ca(2+) rise (calculated as the time from start of stimulation of the cell until the first value above an extrapolated baseline), nadir of initial lowering (difference between the baseline and lowest [Ca(2+)](i) value), and peak height (difference between baseline and the highest [Ca(2+)](i) value of the first calcium peak) in single dispersed beta-cells, in 'single beta-cell within a small cluster', in clusters of medium and large size, and in single dispersed beta-cells from lean mouse and rat. The lag-times for Ca(2+) rise and peak heights were correlated within the pairs of stimulation also in intact ob/ob islets. In summary, despite a large heterogeneity of the 'early [Ca(2+)](i) response' among individual cells, the lag-time for [Ca(2+)](i) rise, the nadir of initial lowering and the height of the first peak response can be identified as cell-specific markers in beta-cells.
Place, publisher, year, edition, pages
Elsevier ScienceDirect , 2003. Vol. 34, no 2, 121-129 p.
Islets of Langerhans, Lag-time, Heterogeneity, Calcium, Cell specific
IdentifiersURN: urn:nbn:se:umu:diva-4884DOI: 10.1016/S0143-4160(03)00027-7PubMedID: 12810054OAI: oai:DiVA.org:umu-4884DiVA: diva2:144154