Cloning, characterization and expression of human LIG1
2001 (English)In: Biochemical and Biophysical Research Communications - BBRC, ISSN 0006-291X, E-ISSN 1090-2104, Vol. 284, no 5, 1155-1161 p.Article in journal (Refereed) Published
Growth factor receptors are frequently amplified and over-expressed in various human cancers. Recently, a Drosophila cell surface protein, Kekkon-1, was found to participate in an epidermal growth factor (EGF) driven negative feedback loop. Kekkon-1 is induced by EGF, binds to the EGF-receptor, and inhibits receptor-mediated signaling. Here, we have searched for human genes with homologies to Kekkon-1 and identified human LIG1. The gene is the human homologue of mouse Lig-1 and is located on chromosome band 3p14, a region frequently deleted in various human cancers. It is predicted to encode a transmembrane cell-surface protein with extracellular leucine-rich repeats and immunoglobulin-like domains. LIG1 mRNA was detected in all tissues analyzed. The highest and lowest relative expression levels were found in brain and spleen, respectively, and differed by more than 200-fold. Taken together, our data are compatible with a role for LIG1 as a growth and tumor suppressor in human tissues.
Place, publisher, year, edition, pages
2001. Vol. 284, no 5, 1155-1161 p.
LIG-1, 3p14, EGF, cancer, tumor suppressor, FISH, real-time PCR
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-5120DOI: 10.1006/bbrc.2001.5092PubMedID: 11414704OAI: oai:DiVA.org:umu-5120DiVA: diva2:144511