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Translocation of virulence determinants is mediated by a surface located protein-lipid complex.
Umeå University, Faculty of Medicine, Molecular Biology.
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Manuscript (Other academic)
Identifiers
URN: urn:nbn:se:umu:diva-5182OAI: oai:DiVA.org:umu-5182DiVA: diva2:144593
Available from: 2006-05-11 Created: 2006-05-11 Last updated: 2010-01-13Bibliographically approved
In thesis
1. Delivery of TypeIII Secreted Toxins by Yersinia pseudotuberculosis: the Role of LcrV, YopD, and Free Lipids in the Translocation Process
Open this publication in new window or tab >>Delivery of TypeIII Secreted Toxins by Yersinia pseudotuberculosis: the Role of LcrV, YopD, and Free Lipids in the Translocation Process
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Bacteria that infect humans and animals face a hard combat with the host´s immune system and in order to establish infection, pathogenic bacteria has evolved mechanisms to avoid being cleared from the host tissue. Many Gram-negatives carry a Type 3 secretion (T3S) system that is used to deliver effector proteins (toxins) into host cells. The toxins exhibit a broad range of intra cellular effects that has in common that they increase the chances of the bacteria to establish infection, multiply in infected tissue or spread to other tissues or hosts. The object of this study was to analyse the mechanisms behind the T3S effectors delivery into target cells. Two bacterial proteins, LcrV and YopD, which are involved in the translocation of effectors were analyzed by mutagenesis. LcrV was found to affect the efficiency of the translocation, probably by determining the size of the pore in the target cell membrane through which the effectors pass. Truncated variants of the multi-functional YopD revealed that defined regions of the protein were important for pore-formation and translocation. Effectors and translocators were demonstrated to form complexes with free acyl chains (lipids) at the bacterial surface. These complexes –termed Yop-lipid complexes, (YLC)– are released from the surface and can act as discrete units that are able to promote translocation of effectors even when separated from the bacterium from which they originate. These findings shed new light on the process of effector translocation by Yersinia and possibly by other gram-negative bacterial pathogens with a similar T3S setup.

Place, publisher, year, edition, pages
Umeå: Molekylärbiologi (Teknisk-naturvetenskaplig fakultet), 2006. 60 p.
Keyword
Yersinia, T3SS, translocation, YopD, LcrV, YLC, fatty acids.
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:umu:diva-800 (URN)91-7264-092-8 (ISBN)
Public defence
2006-06-02, major groove, 6L, NUS, 901 87, Umeå, 10:00 (English)
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Available from: 2006-05-11 Created: 2006-05-11 Last updated: 2009-10-27Bibliographically approved

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