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Molecular epidemiology of streptococcus agalactiae: mobile elements as genetic markers.
Umeå University, Faculty of Medicine, Clinical Microbiology.
2006 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Streptococcus agalactiae, also designated group B streptococcus (GBS), is a Gram-positive coccus, and it is an important pathogen that causes invasive disease in neonates, pregnant adults, and non-pregnant adults with predisposing conditions. The group II intron GBSi1 is one of the major mobile genetic elements identified in S. agalactiae. The aim of this thesis was to characterize the GBSi1 distribution pattern, the population structure, and the influence of serotype- and clone-specific properties on the invasive capacity among clinical invasive and non-invasive isolates of S. agalactiae.

Two additional copies of GBSi1 were identified at sites different from the primarily identified scpB-lmb locus. The distribution of GBSi1 was uneven among different serotypes. Three intron copies were only found in isolates of serotype III, and these targeted all the three identified gene loci. In contrast, a single copy of GBSi1 was found in isolates of serotype II and V and only located at the scpB-lmb locus. Furthermore, at the 5′ flanking region of the scpB-lmb gene locus, a novel 2.1 kb DNA fragment with plasmid features was identified only in intron carrying isolates. This may suggest that GBSi1 once was brought into the S. agalactiae genome by an integrated plasmid.

Multilocus sequence typing was used to characterize totally 314 invasive and non-invasive S. agalactiae isolates collected in Northern and Western Sweden from the years 1988 to 2004. Five major genetic lineages (clonal complexes) were identified among both invasive and non-invasive isolates, including serotype Ia, Ib, and II to V, indicating a clonal population structure of S. agalactiae isolates. A number of genetically highly related isolates were found to express different capsular types, suggesting that capsule switching occurs rather frequently between isolates. Furthermore, non-invasive isolates belonging to the same clonal complexes displayed more heterogeneity in capsule expression as well as in the distribution patterns of mobile genetic elements than invasive isolates. This indicates that less variability is allowed in a highly selective environment such as the blood. All major clonal complexes and serotypes caused invasive disease, although their ability to do so varied greatly. CC17 was significantly associated with neonatal invasive disease; whereas CC19 was equally common among isolates from adult and neonatal disease, despite that both CC17 and CC19 expressed capsular type III. This striking difference seen between CC17 and CC19 suggests that clonal complex associated properties, in addition to capsular type, play important roles in the virulence of S. agalactiae. CC1, a new emerging clone since early 1990s, has caused substantial amount of disease among adults. In addition, mutually exclusive distribution of mobile elements GBSi1 and IS1548 was seen, and they were shown to constitute genetic markers for serotype III CC17 and CC19 isolates, respectively.

Place, publisher, year, edition, pages
Umeå: Klinisk mikrobiologi , 2006. , 73 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1039
Keyword [en]
Streptococcus agalactiae, group II intron (GBSi1), multilocus sequence typing, capsular serotype, population structure
National Category
Microbiology in the medical area
Identifiers
URN: urn:nbn:se:umu:diva-877ISBN: 91-7264-132-0 (print)OAI: oai:DiVA.org:umu-877DiVA: diva2:144850
Public defence
2006-10-20, Betula, 6M, Norrlands Universitetssjukhus, Umeå, 09:00
Opponent
Supervisors
Available from: 2006-09-28 Created: 2006-09-28Bibliographically approved
List of papers
1. An inserted DNA fragment with plasmid features is uniquely associated with the presence of the GBSi1 group II intron in Streptococcus agalactiae.
Open this publication in new window or tab >>An inserted DNA fragment with plasmid features is uniquely associated with the presence of the GBSi1 group II intron in Streptococcus agalactiae.
2003 (English)In: Gene, ISSN 0378-1119, Vol. 312, 305-312 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-5355 (URN)10.1016/S0378-1119(03)00634-6 (DOI)12909368 (PubMedID)
Available from: 2006-09-28 Created: 2006-09-28 Last updated: 2009-10-12Bibliographically approved
2. Multilocus sequence typing of Swedish invasive group B streptococcus isolates indicates a neonatally associated genetic lineage and capsule switching.
Open this publication in new window or tab >>Multilocus sequence typing of Swedish invasive group B streptococcus isolates indicates a neonatally associated genetic lineage and capsule switching.
Show others...
2005 (English)In: Journal of Clinical Microbiology, ISSN 0095-1137, Vol. 43, no 8, 3727-3733 p.Article in journal (Refereed) Published
Identifiers
urn:nbn:se:umu:diva-5356 (URN)10.1128/JCM.43.8.3727-3733.2005 (DOI)16081902 (PubMedID)
Available from: 2006-09-28 Created: 2006-09-28 Last updated: 2009-10-12Bibliographically approved
3. Serotype- and Clonal Complex-Specific Differences in Disease Potential of Streptococcus agalactiae.
Open this publication in new window or tab >>Serotype- and Clonal Complex-Specific Differences in Disease Potential of Streptococcus agalactiae.
(English)Manuscript (Other (popular science, discussion, etc.))
Identifiers
urn:nbn:se:umu:diva-5357 (URN)
Available from: 2006-09-28 Created: 2006-09-28 Last updated: 2010-01-14Bibliographically approved

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CiteExportLink to record
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Citation style
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Output format
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