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Spleno-pancreatic development assessed by 3D molecular imaging
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).
2007 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

The development of different organs and tissues along the gastrointestinal tract, including the pancreas, depends on signalling between the endoderm and the adjacent mesenchyme. The Nkx gene Bapx1 is involved in spatial control of organ-positioning in the spleno-pancreatic region, and deficiency in this gene results in unacceptable proximity of the splenic mesenchyme to the pancreas. This permits agitating signals from the splenic mesenchyme to induce an in vivo (and in vitro) transformation of pancreatic epithelium to a cystic structure with gut like features. Also, wild type splenic mesenchyme is competent to induce a similar transformation. These findings illustrate the importance for strict control of organ positioning during spleno-pancreatic development.

Several growth factors and receptors involved in pancreatic development are activated by protease processing. Some of these growth factors have been implicated as substrates for members of the A Disintegrin And Metalloprotease (ADAM) family. The ADAMs 9, 10, and 17 are expressed during pancreatic development and in the adult pancreas, suggesting a possible role for these ADAMs in pancreatic development and function.

Animal model systems are widely used to investigate gene function during development and disease. However, spatial, molecular, and quantitative phenotype screening in animals is a time consuming effort. Optical Projection Tomography is a 3-dimensional imaging technique that, in combination with improvements in sample preparation and computer processing, can be used to visualize and quantify characteristics of intact adult mouse organs such as the total β-cell content in the pancreas.

Place, publisher, year, edition, pages
Umeå: Umeå centrum för molekylär medicin (UCMM) , 2007. , 94 p.
Series
Umeå University medical dissertations, ISSN 0346-6612 ; 1064
Keyword [en]
Cell biology, Spleen, Pancreas, Imaging, Development
Keyword [sv]
Cellbiologi
Research subject
Developmental Biology
Identifiers
URN: urn:nbn:se:umu:diva-987ISBN: 91-7264-203-3 (print)OAI: oai:DiVA.org:umu-987DiVA: diva2:145301
Public defence
2007-02-15, Betula, Byggnad 6M, Umeå, 09:00 (English)
Opponent
Supervisors
Available from: 2007-01-31 Created: 2007-01-31 Last updated: 2009-05-14Bibliographically approved
List of papers
1. Spleen versus pancreas: strict control of organ interrelationship revealed by analyses of Bapx1-/- mice.
Open this publication in new window or tab >>Spleen versus pancreas: strict control of organ interrelationship revealed by analyses of Bapx1-/- mice.
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2006 (English)In: Genes & Development, ISSN 0890-9369, Vol. 20, no 16, 2208-2213 p.Article in journal (Refereed) Published
Keyword
Animals, Endoderm/metabolism, Female, Hedgehog Proteins, Homeodomain Proteins/genetics/*physiology, Male, Mesoderm/metabolism, Mice, Mice; Inbred C57BL, Mice; Inbred CBA, Models; Animal, Mutation, Organogenesis, Pancreas/*embryology, Spleen/*embryology, Trans-Activators/metabolism, Transcription Factors/genetics/*physiology
Identifiers
urn:nbn:se:umu:diva-5823 (URN)10.1101/gad.381906 (DOI)16912273 (PubMedID)
Available from: 2007-11-30 Created: 2007-11-30Bibliographically approved
2. Developmental expression of metalloproteases ADAM 9, 10, and 17 becomes restricted to divergent pancreatic compartments.
Open this publication in new window or tab >>Developmental expression of metalloproteases ADAM 9, 10, and 17 becomes restricted to divergent pancreatic compartments.
2005 (English)In: Developmental Dynamics, ISSN 1058-8388, Vol. 232, no 4, 1105-1114 p.Article in journal (Refereed) Published
Keyword
Animals, Female, Gene Expression Regulation; Developmental/*physiology, Islets of Langerhans/cytology/*embryology, Male, Metalloendopeptidases/*biosynthesis, Mice, Mice; Inbred CBA, Organ Specificity/physiology
Identifiers
urn:nbn:se:umu:diva-5826 (URN)10.1002/dvdy.20259 (DOI)15739225 (PubMedID)
Available from: 2007-11-30 Created: 2007-11-30Bibliographically approved
3. Tomographic molecular imaging and 3D quantification within adult mouse organs.
Open this publication in new window or tab >>Tomographic molecular imaging and 3D quantification within adult mouse organs.
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2007 (English)In: Nature Methods, ISSN 1548-7091, Vol. 4, no 1, 31-33 p.Article in journal (Refereed) Published
Keyword
Animals, Diabetes Mellitus; Type 1/*diagnosis/physiopathology, Disease Models; Animal, Disease Progression, Image Processing; Computer-Assisted/*methods, Imaging; Three-Dimensional/*methods, Islets of Langerhans/*pathology, Mice, Pancreas/*pathology, Sensitivity and Specificity, Tomography/*methods
Identifiers
urn:nbn:se:umu:diva-5821 (URN)10.1038/nmeth985 (DOI)17143281 (PubMedID)
Available from: 2007-11-30 Created: 2007-11-30 Last updated: 2009-05-14Bibliographically approved

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CiteExportLink to record
Permanent link

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Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
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  • de-DE
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  • fi-FI
  • nn-NO
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Output format
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