Change search
ReferencesLink to record
Permanent link

Direct link
Haematopoietic progenitor cells utilise conventional PKC to suppress PKB/Akt activity in response to c-Kit stimulation
Umeå University, Faculty of Medicine, Department of Medical Biosciences. Umeå University, Faculty of Medicine, Department of Medical Biosciences, Pathology.
Experimental Clinical Chemistry, Lund University, Malmö University Hospital, Malmö.
Umeå University, Faculty of Medicine, Umeå Centre for Molecular Medicine (UCMM).
Experimental Clinical Chemistry, Lund University, Malmö University Hospital, Malmö.
Show others and affiliations
2007 (English)In: British Journal of Haematology, ISSN 0007-1048, E-ISSN 1365-2141, Vol. 136, no 2, 260-268 p.Article in journal (Refereed) Published
Abstract [en]

Receptor tyrosine kinase (RTK) c-Kit signalling is crucial for the proliferation, survival and differentiation of haematopoietic stem cells (HSCs). To further understand the mechanisms underlying these events we explored how the downstream mediators interact. The present study investigated the function of conventional protein kinase Cs (c-PKC) in c-Kit mediated signalling pathways in HSC-like cell lines. This analysis supported earlier findings, that steel factor (SF) activates c-PKC, extracellular signal-regulated kinase (Erk) and protein kinase B (PKB). The present results were consistent with an important role of c-PKC in the positive activation of Erk and for proliferation. Further, it was observed that c-PKC negatively regulated PKB activity upon SF stimulation, indicating that c-PKC acts as a suppressor of c-Kit signalling. Finally, these observations were extended to show that c-PKC mediated the phosphorylation of the endogenous c-Kit receptor on serine 746, resulting in decreased overall tyrosine phosphorylation of c-Kit upon SF stimulation. This report showed that this specific feedback mechanism of c-PKC mediated phosphorylation of the c-Kit receptor has consequences for both proliferation and survival of HSC-like cell lines.

Place, publisher, year, edition, pages
Oxford: Blackwell Scientific , 2007. Vol. 136, no 2, 260-268 p.
Keyword [en]
blotting, western/methods, cell line, cell proliferation, cell survival, enzyme activation, extracellular signal-regulated map kinases/metabolism, flow cytometry, hematopoietic stem cells/enzymology/*metabolism, humans, immunoprecipitation, phosphorylation, protein kinase c/*metabolism, proto-oncogene proteins c-akt/*metabolism, proto-oncogene proteins c-kit/*metabolism, signal transduction/*physiology, stem cell factor/metabolism/pharmacology
URN: urn:nbn:se:umu:diva-5916DOI: 10.1111/j.1365-2141.2006.06434.xPubMedID: 17156394OAI: diva2:145584
Available from: 2008-01-10 Created: 2008-01-10 Last updated: 2011-03-29Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Edling, Charlotte E.Carlsson, LeifPalmer, Ruth H.Hallberg, Bengt
By organisation
Department of Medical BiosciencesPathologyUmeå Centre for Molecular Medicine (UCMM)Umeå Centre for Molecular Pathogenesis (UCMP)
In the same journal
British Journal of Haematology

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 43 hits
ReferencesLink to record
Permanent link

Direct link