Expression and properties of sorting nexin 9 in dynamin-mediated endocytosis
2005 (English)In: Methods in Enzymology, ISSN 0076-6879, Vol. 404, 545-556 p.Article in journal (Refereed) Published
Sorting nexin 9 (SNX9) is identified as an important regulator of dynamin function in clathrin-mediated endocytosis. SNX9 recruits dynamin to the plasma membrane and promotes its GTPase activity, resulting in membrane constriction and ultimate transport vesicle scission. This chapter describes procedures to express recombinant SNX9, to biochemically characterize the cytosolic complex between SNX9 and dynamin, and to identify additional interacting partners of SNX9. Assays are presented to investigate the requirements for SNX9-dependent membrane recruitment of dynamin in vitro and in vivo.
Place, publisher, year, edition, pages
Elsevier, 2005. Vol. 404, 545-556 p.
Carrier Proteins/*biosynthesis/*physiology, Cytosol/metabolism, Dynamins/*physiology, Endocytosis/*physiology, Glutathione Transferase/genetics, Humans, K562 Cells, Liposomes/metabolism, Protein Interaction Mapping, Recombinant Fusion Proteins/biosynthesis, Vesicular Transport Proteins
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:umu:diva-6537DOI: 10.1016/S0076-6879(05)04048-6ISI: 000234565700048PubMedID: 16413299OAI: oai:DiVA.org:umu-6537DiVA: diva2:146206
ISBN: 978-0-12-405882-82007-12-132007-12-132013-07-10Bibliographically approved