Cytotoxicity of albebetin oligomers depends on cross-beta-sheet formation
2006 (English)In: FEBS Letters, ISSN 0014-5793, E-ISSN 1873-3468, Vol. 580, no 10, 2451-2457 p.Article in journal (Refereed) Published
Prefibrillar cytotoxicity was suggested as a common amyloid characteristic. We showed two types of albebetin prefibrillar oligomers are formed during incubation at pH 7.3. Initial round-shaped oligomers consist of 10–15 molecules determined by atomic force microscopy, do not bind thioflavin-T and do not affect viability of granular neurons and SH-SY5Y cells. They are converted into ca. 30–40-mers possessing cross-β-sheet and reducing viability of neuronal cells. Neither monomers nor fibrils possess cytotoxicity. We suggest that oligomeric size is important for stabilising cross-β-sheet core critical for cytotoxicity. As albebetin was used as a carrier-protein for drug delivery, examination of amyloidogenicity is required prior polypeptide biomedical applications.
Place, publisher, year, edition, pages
2006. Vol. 580, no 10, 2451-2457 p.
Amyloid; Oligomers; Cytotoxicity; Atomic force microscopy
Medical Biotechnology (with a focus on Cell Biology (including Stem Cell Biology), Molecular Biology, Microbiology, Biochemistry or Biopharmacy)
IdentifiersURN: urn:nbn:se:umu:diva-6632DOI: 10.1016/j.febslet.2006.03.074PubMedID: 16638570OAI: oai:DiVA.org:umu-6632DiVA: diva2:146302