Telomere length and correlation with histopathogenesis in B-cell leukemias/lymphomas
2007 (English)In: European Journal of Haematology, ISSN 0902-4441, E-ISSN 1600-0609, Vol. 78, no 4, 283-289 p.Article in journal (Refereed) Published
Telomere length was recently reported to correlate with cellular origin of B-cell malignancies in relation to the germinal center (GC). In this report, we measured telomere length by quantitative-PCR in 223 B-cell lymphomas/leukemias and correlated results with immunoglobulin (Ig) mutation status and immunostainings for GC/non-GC subtypes of diffuse large B-cell lymphoma (DLBCL). Shortest telomeres were found in Ig-unmutated chronic lymphocytic leukemia (CLL) [median telomere to single copy gene value (T/S) 0.33], differing significantly to Ig-mutated CLL (0.63). Contrary to this, mantle cell lymphomas (MCLs) exhibited similar telomere lengths regardless of Ig mutation status (0.47). Telomere length differed significantly between GC-like (0.73) and non-GC-like DLBCLs (0.43), and follicular lymphomas (FLs) had shorter telomeres (0.53) than GC-DLBCL. Hairy cell leukemias, which display Ig gene intraclonal heterogeneity, had longer telomeres (0.62) than FLs and non-GC-DLBCL, but shorter than GC-DLBCL. We conclude that although DLBCL and CLL subsets can be clearly distinguished, telomere length reflects many parameters and may not simply correlate with GC-related origin.
Place, publisher, year, edition, pages
2007. Vol. 78, no 4, 283-289 p.
DNA Mutational Analysis, Germinal Center/*pathology, Humans, Immunoglobulins/*genetics, Leukemia; B-Cell/diagnosis/*genetics, Lymphoma; B-Cell/diagnosis/*genetics, Mutation, Reverse Transcriptase Polymerase Chain Reaction/methods, Sensitivity and Specificity, Telomere/*genetics
Cell and Molecular Biology
Research subject Pathology
IdentifiersURN: urn:nbn:se:umu:diva-7565DOI: 10.1111/j.1600-0609.2007.00817.xPubMedID: 17286609OAI: oai:DiVA.org:umu-7565DiVA: diva2:147236