Interleukin-1alpha downregulates extracellular-superoxide dismutase in human corneal keratoconus stromal cells
2007 (English)In: Molecular Vision, ISSN 1090-0535, E-ISSN 1090-0535, Vol. 13, 1285-1290 p.Article in journal (Other academic) Published
PURPOSE: The purpose of this investigation was to elucidate the regulation of corneal extracellular superoxide dismutase (SOD3) synthesis in keratoconus. We compared the basal and cytokine-regulated SOD3 synthesis in cultured human stromal cells from keratoconus corneas to stromal cells from normal and bullous keratopathy corneas.
METHODS: Keratocyte cultures were obtained from patients undergoing corneal transplantation for keratoconus and bullous keratopathy, and from healthy donor corneas. The cell lines obtained were cultured until near confluence and interleukin-1alpha, interleukin-6, transforming growth factor beta, or platelet derived growth factor were added to the media. The phenotypes of the cultured cells were assessed by immunocytochemical expression of alpha-smooth muscle actin and CD34. SOD3 protein contents were determined in the culture media with ELISA after 24, 48, 72, and 96 h.
RESULTS: Interleukin-1alpha had an inhibitory effect on SOD3 synthesis exclusively in the keratoconus cultures (p<0.01). Platelet derived growth factor induced a reduction in SOD3 synthesis in all groups (p<0.05).
CONCLUSIONS: Here, we demonstrate that cultured keratoconus stromal cells respond with a reduced SOD3 synthesis to interleukin-1alpha, which is not the case in corresponding normal or bullous keratopathy cells. Since interleukin-1alpha is upregulated in corneal trauma and inflammation, keratoconus corneas may muster an insufficient oxidative defense under such conditions.
Place, publisher, year, edition, pages
2007. Vol. 13, 1285-1290 p.
Cell Death/drug effects, Cells; Cultured, Corneal Stroma/cytology/*drug effects/*enzymology/pathology, Down-Regulation/*drug effects, Fibroblasts/drug effects/enzymology, Humans, Immunohistochemistry, Interleukin-1alpha/*pharmacology, Keratoconus/*enzymology/*pathology, Superoxide Dismutase/biosynthesis/*metabolism
Research subject Ophtalmology
IdentifiersURN: urn:nbn:se:umu:diva-8133PubMedID: 17679946Local ID: 744OAI: oai:DiVA.org:umu-8133DiVA: diva2:147804