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Donor-donor energy migration (DDEM) as a tool for studying aggregation in lipid phases
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
2001 (English)In: SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR, ISSN 1386-1425, Vol. 57, no 9, 1839-45 p.Article in journal (Refereed) Published
Abstract [en]

A BODIPY(R)-labelled sulfatide (N-(BODIPY(R)- FL-pentanoyl) -galactosylcerebroside-sulfate, hereafter abbreviated as BD-Sulfatide) was solubilised at different concentrations in lipid vesicles of 1,2-dioleoyl-sn -glycero-3-phosphocholine (DOPC). Time-correlated single photon counting experiments show that the fluorescence relaxation is mono-exponential (with a lifetime of 6.5 ns) at molar ratios of BD-Sulfatide: DOPC that are less than 1:100. The fluorescence steady-state anisotropy decreases monotonously at molar ratios smaller than 1:1000, which is compatible with donor-donor energy migration (DDEM) among the BODIPY(R) groups. A model that assumes DDEM across the lipid bilayers, as well as in their planes, was used to analyse the time-resolved fluorescence anisotropy. Only two parameters appear in the model namely; the bilayer thickness (d) and the average number density (C-2) distribution of BD-Sulfatide in the lipid bilayers. The extracted d-values vary between 35 and 40 Angstrom, which is about the reported thickness of a bilayer of DOPC (38 Angstrom). Hence, the BODIPY(R) groups are preferentially located in the water-lipid interface. At low concentration the experimental C-2-values and those independently calculated are in good agreement, while the experimental values gradually become lower with increasing BD-Sulfatide concentration. These results are compatible with an aggregation of the sulfatides and self-quenching of BODIPY(R), which is clearly established at higher concentrations of the BD-Sulfatide.

Place, publisher, year, edition, pages
2001. Vol. 57, no 9, 1839-45 p.
Keyword [en]
URN: urn:nbn:se:umu:diva-8649DOI: doi:10.1016/S1386-1425(01)00411-5OAI: diva2:148320
Available from: 2008-05-06 Created: 2008-05-06 Last updated: 2011-01-13Bibliographically approved

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