Two-photon excitation and time-resolved fluorescence: I. The proper response function for analysing single-photon counting experiments
2002 (English)In: Chemical Physics Letters, Vol. 354, no 5-6, 367-75 p.Article in journal (Refereed) Published
An accurate instrumental response function is needed to deconvolute fluorescence data obtained by time-correlated single-photon counting (TCSPC) upon multi-photon excitation. Hitherto the response function was obtained by measuring Rayleigh scattering (RS) from colloidal solutions, as is also used in one-photon excited fluorescence. We show that hyper Rayleigh scattering (HRS) provides a better choice for deconvolution of fluorescence decays, as obtained by TCSPC and two-photon excitation (TPE). The one- and two-photon response functions were monitored as RS and HRS from colloidal gold particles at 800 and 400 nm, respectively.
Place, publisher, year, edition, pages
2002. Vol. 354, no 5-6, 367-75 p.
IdentifiersURN: urn:nbn:se:umu:diva-8723DOI: doi:10.1016/S0009-2614(02)00141-0OAI: oai:DiVA.org:umu-8723DiVA: diva2:148394