Hyper Rayleigh Scattering Yields Improved Response Function in Analysing 2-Photon Excited Fluorescence
2002 (English)In: Journal of Fluorescence, ISSN 1053-0509 (Print) 1573-4994 (Online), Vol. 12, no 3-4, 481-4 p.Article in journal (Refereed) Published
An accurate instrumental response function is needed to conclusively deconvolute fluorescence data based on time-correlated single-photon counting (TCSPC) and multiphoton excitation. Routinely the response function is measured as Rayleigh scattering (RS) from a colloidal solution, even if the excitation is a multiphoton event. Present work demonstrates that a response function obtained as hyper Rayleigh scattering (HRS) provides a better choice for deconvolution of 2-photon excited fluorescence decays. The 1- and 2-photon response functions were monitored as RS and HRS from colloidal gold particles at 800 and 400 nm, respectively.
Place, publisher, year, edition, pages
2002. Vol. 12, no 3-4, 481-4 p.
Two-photon-excited fluorescence, hyper Rayleigh scattering, response function - time-correlated single-photon counting
IdentifiersURN: urn:nbn:se:umu:diva-8740DOI: doi:10.1023/A:1021378313407OAI: oai:DiVA.org:umu-8740DiVA: diva2:148411