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High-Performance Liquid Chromatography Analysis of Spectrin Oligomerization
Umeå University, Faculty of Science and Technology, Chemistry.
2001 (English)In: Analytical Biochemistry, Vol. 292, no 2, 222-7 p.Article in journal (Refereed) Published
Abstract [en]

Gel filtration chromatography has been used to analyze the oligomerization of human erythrocyte spectrin. By applying an exponentially modified Gaussian function we have been able to resolve overlapping elution peaks. From these peaks it was possible to calculate the equilibrium composition of each spectrin concentration and thus also the dissociation constants describing the oligomeric process. The determined dissociation constants for tetramer formation (1.3 M) and for hexamer formation (24 M) agree well with other measurements.

Place, publisher, year, edition, pages
2001. Vol. 292, no 2, 222-7 p.
URN: urn:nbn:se:umu:diva-8777OAI: diva2:148448
Available from: 2008-02-12 Created: 2008-02-12 Last updated: 2011-01-13Bibliographically approved

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Backman, Lars
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