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Development of a high-performance liquid chromatography carbon column based method for the fractionation of dioxin-like polychlorinated biphenyls
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
2002 (English)In: Journal of Chromatography A, Vol. 962, no 1-2, 79-93 p.Article in journal (Refereed) Published
Abstract [en]

A method to separate polychlorinated biphenyls (PCBs) by using high-performance liquid chromatography (HPLC) was developed. The HPLC column was packed with Amoco PX-21 activated carbon dispersed on octadecylsilane (ODS). The separation was carried out by gradient elution with n-hexane–dichloromethane and toluene in the forward direction followed by reversed elution with toluene. The results show that this HPLC method is useful for the separation of PCBs according to the number of substituted ortho chlorine atoms attached to the biphenyl structure. Average recoveries for a number of individual di-ortho PCBs, mono-ortho PCBs, and non-ortho PCBs in three selected elution windows were 97, 92, and 96%, respectively. Clophen A50, a herring sample, and a cod liver oil sample were fractionated on the column and the analytical results are compared with data from the literature. The method presented here is useful for quantitative separations of mono-ortho PCBs as well as non-ortho PCBs which have been assigned toxic equivalency factors by the World Health Organisation.

Place, publisher, year, edition, pages
2002. Vol. 962, no 1-2, 79-93 p.
Keyword [en]
Polychlorinated biphenyls, Clophen A50
URN: urn:nbn:se:umu:diva-8837DOI: doi:10.1016/S0021-9673(02)00443-0OAI: diva2:148508
Available from: 2008-02-18 Created: 2008-02-18 Last updated: 2011-01-13Bibliographically approved

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