Change search
ReferencesLink to record
Permanent link

Direct link
Structure dependent induction of CYP1A by polychlorinated biphenyls in hepatocytes of male castrated pigs
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
Show others and affiliations
2000 (English)In: Chemosphere, Vol. 41, no 10, 1697-1708 p.Article in journal (Refereed) Published
Abstract [en]

Hepatocytes cultures prepared from castrated pig hepatocytes (Great YorkshirexDutch Landrace), as a model for human liver, were used to study the effect of twenty polychlorinated biphenyls (PCBs) on CYP1A activity, measured as the dealkylation of either ethoxyresorufin or methoxyresorufin. The selection of the PCBs was based on their differences in physico-chemical properties. The non-ortho and mono-ortho substituted PCBs were the most potent CYP1A inducers in pig hepatocytes. In addition, several multiple-ortho substituted congeners, with five or more chlorine atoms, were inducers of CYP1A activity as well. Their relative effect potencies (REP) were proximately 10,000 times lower than the most potent congener, 3,3',4,4',5 PeCB (PCB#126). Using partial least-squares (PLS) modeling, predictions of CYP1A activity could be made for all tetra to hepta substituted congeners. Several multiple-ortho substituted PCBs, which are highly abundant in the biotic and abiotic environment, have been found to induce CYP1A activity in pig hepatocytes. Because induction of CYP1A activity is used as biomarker for Ah-receptor mediated responses, it is suggested to include these congeners in future risk assessment.

Place, publisher, year, edition, pages
2000. Vol. 41, no 10, 1697-1708 p.
Keyword [en]
CYPIA, PCBs, Pigs hepatocytes, TEFs
URN: urn:nbn:se:umu:diva-8940DOI: doi:10.1016/S0045-6535(00)00013-8OAI: diva2:148611
Available from: 2008-02-22 Created: 2008-02-22 Last updated: 2011-01-14Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Tysklind, MatsAndersson, Patrik
By organisation

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 27 hits
ReferencesLink to record
Permanent link

Direct link