Change search
ReferencesLink to record
Permanent link

Direct link
Application of orthogonal signal correction to minimise the effects of physical and biological variation in high resolution 1H NMR spectra of biofluids
Show others and affiliations
2002 (English)In: Analyst, Vol. 127, no 10, 1283-8 p.Article in journal (Refereed) Published
Abstract [en]

1H nuclear magnetic resonance (NMR)-based metabonomics is a well-established technique used to analyse and interpret complex multiparametric metabolic data, and has a wide number of applications in the development of pharmaceuticals. However, interpretation of biological data can be confounded by extraneous variation in the data such as fluctuations in either experimental conditions or in physiological status. Here we have shown the novel application of a data filtering method, orthogonal signal correction (OSC), to biofluid NMR data to minimise the influence of inter- and intra-spectrometer variation during data acquisition, and also to minimise innate physiological variation. The removal of orthogonal variation exposed features of interest in the NMR data and facilitated interpretation of the derived multivariate models. Furthermore, analysis of the orthogonal variation provided an explanation of the systematic analytical/biological changes responsible for confounding the original NMR data.

Place, publisher, year, edition, pages
2002. Vol. 127, no 10, 1283-8 p.
URN: urn:nbn:se:umu:diva-9103OAI: diva2:148774
Note from: 2008-02-29 Created: 2008-02-29 Last updated: 2011-01-13Bibliographically approved

Open Access in DiVA

No full text

Other links

Search in DiVA

By author/editor
Antti, Henrik
By organisation

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Total: 26 hits
ReferencesLink to record
Permanent link

Direct link