D1′ centers are less efficient than normal photosystem II centers
2001 (English)In: FEBS Letters, Vol. 505, no 1, 113-7 p.Article in journal (Refereed) Published
One prominent difference between the photosystem II (PSII) reaction center protein D1′ in Synechocystis 6803 and normal D1 is the replacement of Phe-186 in D1 with leucine in D1′. Mutants of Synechocystis 6803 producing only D1′, or containing engineered D1 proteins with Phe-186 substitutions, were analyzed by 77 K fluorescence emission spectra, chlorophyll a fluorescence induction yield and decay kinetics, and flash-induced oxygen evolution. Compared to D1-containing PSII centers, D1′ centers exhibited a 50% reduction in variable chlorophyll a fluorescence yield, while the flash-induced O2 evolution pattern was unaffected. In the F186 mutants, both the P680+/QA− recombination and O2 oscillation pattern were noticeably perturbed.
Place, publisher, year, edition, pages
2001. Vol. 505, no 1, 113-7 p.
D1; D1′; Photosystem II; psbA; Synechocystis
IdentifiersURN: urn:nbn:se:umu:diva-9597DOI: doi:10.1016/S0014-5793(01)02794-6OAI: oai:DiVA.org:umu-9597DiVA: diva2:149268