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Gramicidin A interaction at a dioleoyl phosphatidylcholine monolayer on a mercury drop electrode
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
2003 (English)In: Analytical and Bioanalytical Chemistry, Vol. 375, no 3, 350-5 p.Article in journal (Refereed) Published
Abstract [en]

A biosensor where the sensing surface is a fluid dioleyl phosphatidylcholine monolayer (DOPC) deposited on a mercury drop was used. The lipid monolayer was held in 0.1 M NaCl and a concentration of gramicidin A in the range 0-12 nM was used. Electrochemical impedance spectroscopy in the frequency range 0.1-65 kHz was employed to investigate how the defect-free monolayer responds to interactions of gramicidin A in solution.

The data was analyzed both with multivariate data analysis and classical electrochemical methods. The principal component analysis of the resulting impedance spectra gave a linear dependence on the concentration of gramicidin A. An increasing permittivity was observed in the low-frequency regime with increasing concentration of gramicidin A in solution.

Place, publisher, year, edition, pages
2003. Vol. 375, no 3, 350-5 p.
Keyword [en]
Phospholipid monolayer, Gramicidin A, DOPC, Impedance spectroscopy, Multivariate analysis
Identifiers
URN: urn:nbn:se:umu:diva-9963DOI: doi:10.1007/s00216-002-1697-9OAI: oai:DiVA.org:umu-9963DiVA: diva2:149634
Available from: 2008-06-03 Created: 2008-06-03 Last updated: 2011-01-13Bibliographically approved

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Lindholm-Sethson, Britta

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