An environmental-sensitive BODIPY®-derivative with bioapplication: spectral and photophysical properties
2003 (English)In: Journal of Fluorescence, ISSN 1053-0509 (Print) 1573-4994 (Online), Vol. 13, no 5, 379-84 p.Article in journal (Refereed) Published
A previously synthesised derivative of BODIPY aimed for sulfhydryl specific labelling of cysteine residues in proteins was studied. The spectral and photophysical properties of this derivative, N-(4,4-difluoro-1,3,5,7-tetramethyl-4-bora-3a,4a-diaza-s-indacene-2-yl) iodoacetamide (NBDY) were characterised, and found to be considerably different from those of commonly used derivatives of BODIPY, e.g. N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-yl)methyl iodoacetamide. The absorption and fluorescence spectra, as well as fluorescence lifetimes and quantum yields of NBDY are quite sensitive to solvent properties. The fluorescence is effectively quenched by I– when NBDY is free in water or attached to Cys in different mutants of plasminogen activator inhibitor type 2 (PAI-2). A ground-state dimer forms when two NBDY groups are closely spaced in plasminogen activator inhibitor type 1 (PAI-1).
Place, publisher, year, edition, pages
2003. Vol. 13, no 5, 379-84 p.
BODIPY, donor–donor energy migration, homotransfer, dimers, quenching
IdentifiersURN: urn:nbn:se:umu:diva-10060DOI: doi:10.1023/A:1026156605013OAI: oai:DiVA.org:umu-10060DiVA: diva2:149731