Enzyme-Linked Immunosorbent Assay for Screening Dioxin Soil Contamination by Uncontrolled Combustion During Informal Recycling in Slums
2008 (English)In: Environmental Toxicology and Chemistry, ISSN 0730-7268, Vol. 27, no 11, 2224-2232 p.Article in journal (Refereed) Published
Uncontrolled combustion due to garbage recycling is a widespread activity among slum dwellers in distressed economy countries, and has been indicated as a major source of dioxin contamination. However, due to the high cost and complexity of gas chromatography-high resolution mass spectrometry (GC-HRMS) analysis, the magnitude of the problem remains largely unknown. This work describes a first approach towards the use of a dioxin antibody-based enzyme-linked immunosorbent assay (ELISA) as the basis for a sustainable, simple, and low cost monitoring program to assess the toxicological impact of uncontrolled combustion in slums. A panel of sixteen samples was analyzed by GC-HRMS and ELISA on split extracts. Close to 20 % of the analyzed samples showed dioxin concentrations up to almost twice the guidance level for residential soil in several countries, pointing out the need for performing a large-scale monitoring program. Despite the potential for variations in dioxin congener distribution due to the mixed nature of the incinerated material, there was a good correlation between the toxic equivalents as determined by GC-HRMS and ELISA. Furthermore, an inter-laboratory ELISA validation showed that the capacity to perform the dioxin ELISA was successfully transferred between laboratories. It was concluded that the ELISA method performed very well as a screening tool to prioritize samples for instrumental analysis, which allows cutting down costs significantly.
Place, publisher, year, edition, pages
John Wiley & Sons, 2008. Vol. 27, no 11, 2224-2232 p.
Polychlorinated dibenzo-p-dioxins/furans, immunoassays, dioxin, slum contamination
IdentifiersURN: urn:nbn:se:umu:diva-10096DOI: 10.1897/07-660.1PubMedID: 18522475OAI: oai:DiVA.org:umu-10096DiVA: diva2:149767