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Enzyme-Linked Immunosorbent Assay for Screening Dioxin Soil Contamination by Uncontrolled Combustion During Informal Recycling in Slums
Catedra de Bioquímica, Faculty of Chemistry, University de la República, Montevideo, Uruguay.
Umeå University, Faculty of Science and Technology, Department of Chemistry. Department of Entomology and Cancer Research Center, University of California, Davis, California 95616, USA.
Department of Entomology and Cancer Research Center, University of California, Davis, California 95616, USA.
Umeå University, Faculty of Science and Technology, Department of Chemistry.
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2008 (English)In: Environmental Toxicology and Chemistry, ISSN 0730-7268, Vol. 27, no 11, 2224-2232 p.Article in journal (Refereed) Published
Abstract [en]

Uncontrolled combustion due to garbage recycling is a widespread activity among slum dwellers in distressed economy countries, and has been indicated as a major source of dioxin contamination. However, due to the high cost and complexity of gas chromatography-high resolution mass spectrometry (GC-HRMS) analysis, the magnitude of the problem remains largely unknown. This work describes a first approach towards the use of a dioxin antibody-based enzyme-linked immunosorbent assay (ELISA) as the basis for a sustainable, simple, and low cost monitoring program to assess the toxicological impact of uncontrolled combustion in slums. A panel of sixteen samples was analyzed by GC-HRMS and ELISA on split extracts. Close to 20 % of the analyzed samples showed dioxin concentrations up to almost twice the guidance level for residential soil in several countries, pointing out the need for performing a large-scale monitoring program. Despite the potential for variations in dioxin congener distribution due to the mixed nature of the incinerated material, there was a good correlation between the toxic equivalents as determined by GC-HRMS and ELISA. Furthermore, an inter-laboratory ELISA validation showed that the capacity to perform the dioxin ELISA was successfully transferred between laboratories. It was concluded that the ELISA method performed very well as a screening tool to prioritize samples for instrumental analysis, which allows cutting down costs significantly.

Place, publisher, year, edition, pages
John Wiley & Sons, 2008. Vol. 27, no 11, 2224-2232 p.
Keyword [en]
Polychlorinated dibenzo-p-dioxins/furans, immunoassays, dioxin, slum contamination
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:umu:diva-10096DOI: 10.1897/07-660.1PubMedID: 18522475OAI: oai:DiVA.org:umu-10096DiVA: diva2:149767
Available from: 2008-10-21 Created: 2008-10-21 Last updated: 2013-09-09Bibliographically approved

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