Polymer-based monolithic microcolumns for hydrophobic interaction chromatography of proteins.
2006 (English)In: Journal of Separation Science, ISSN 1615-9306, Vol. 29, no 1, 25-32 p.Article in journal (Refereed) Published
Monolithic capillary columns for hydrophobic interaction chromatography (HIC) have been prepared by thermally initiated, single-step in situ polymerization of mixtures of monovinyl monomers including butyl methacrylate and/or 2-hydroxyethyl methacrylate, with a divinyl crosslinker glycerol dimethacrylate or 1,4-butanediol dimethacrylate using two different porogen systems. Two porogenic solvent mixtures were used; one "hydrophilic", consisting of water, butanediol, and propanol, and one "hydrophobic," comprising dodecanol and cyclohexanol. The porous structures of the monoliths were characterized and their performance was demonstrated with a separation of a mixture of myoglobin, ribonuclease A, and lysozyme under conditions typical of HIC.
Place, publisher, year, edition, pages
2006. Vol. 29, no 1, 25-32 p.
Capillary, Column, HIC, Hydrophobic interaction chromatography, Monolith, Protein separation
IdentifiersURN: urn:nbn:se:umu:diva-11762DOI: doi:10.1002/jssc.200500239OAI: oai:DiVA.org:umu-11762DiVA: diva2:151433