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Tryptophan-BODIPY: A versatile donor-acceptor pair for probing generic changes of intraprotein distances
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
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2006 (English)In: Physical Chemistry Chemical Physics, ISSN 1463-9076, Vol. 8, no 26, 3130-40 p.Article in journal (Refereed) Published
Abstract [en]

We demonstrate that Tryptophan (Trp) and N-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-yl)methyl iodoacetamide (BODIPY) is a suitable donor-acceptor (D-A) pair for intraprotein distance measurements, applicable to the study of protein folding. The suitability of the Trp-BODIPY electronic energy transfer is exemplified on the extensively-characterised two-state protein, S6, from Thermus thermophilus. This protein has proved to be useful for the elucidation of folding cooperativity and nucleation, as well as the changes upon induction of structural transitions. For a comprehensive structural coverage, BODIPY molecules were anchored by Cys insertions at four different positions on the S6 surface. Trp residues at position 33 or 62 acted as donors of electronic energy to the BODIPY groups. None of the D-A pairs show any detectable difference in the folding kinetics (or protein stability), which supports the notion that the two-state transition of S6 is a highly concerted process. Similar results are obtained for mutants affecting the N- and C-terminus. The kinetic analyses indicate that changes of the transition state occur through local unfolding of the native state, rather than by a decrease of the folding cooperativity. The distances obtained from the analysis of the time-resolved fluorescence experiments in the native state were compared to those calculated from X-ray structure. As an additional measure, molecular dynamics simulations of the different protein constructs were performed to account for variability in the BODIPY location on the protein surface. The agreement between fluorescence and X-ray data is quite convincing, and shows that energy transfer measurements between Trp and BODIPY can probe distances between ca. 17 to 34 A, with an error better than 10%.

Place, publisher, year, edition, pages
2006. Vol. 8, no 26, 3130-40 p.
Keyword [en]
Boron Compounds/analysis/*chemistry, Electron Transport, Fluorescent Dyes/analysis/chemistry, Molecular Probe Techniques, Protein Conformation, Reproducibility of Results, Ribosomal Protein S6/*chemistry/*ultrastructure, Sensitivity and Specificity, Spectrometry; Fluorescence/*methods, Tryptophan/analysis/*chemistry
Identifiers
URN: urn:nbn:se:umu:diva-11790DOI: doi:10.1039/b601313aPubMedID: 16804615OAI: oai:DiVA.org:umu-11790DiVA: diva2:151461
Available from: 2007-06-26 Created: 2007-06-26 Last updated: 2011-01-11Bibliographically approved

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Zdunek, JanuszJohansson, Lennart B-Å

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