Blueberry- and spirulina-enriched diets enhance striatal dopamine recovery and induce a rapid, transient microglia activation after injury of the rat nigrostriatal dopamine system.
2005 (English)In: Experimental Neurology, ISSN 0014-4886, Vol. 196, no 2, 298-307 p.Article in journal (Refereed) Published
Neuroinflammation plays a critical role in loss of dopamine neurons during brain injury and in neurodegenerative diseases. Diets enriched in foods with antioxidant and anti-inflammatory actions may modulate this neuroinflammation. The model of 6-hydroxydopamine (6-OHDA) injected into the dorsal striatum of normal rats, causes a progressive loss of dopamine neurons in the ventral mesencephalon. In this study, we have investigated the inflammatory response following 6-OHDA injected into the striatum of adult rats treated with diet enriched in blueberry or spirulina. One week after the dopamine lesion, a similar size of dopamine degeneration was found in the striatum and in the globus pallidus in all lesioned animals. At 1 week, a significant increase in OX-6- (MHC class II) positive microglia was found in animals fed with blueberry- and spirulina-enriched diets in both the striatum and the globus pallidus. These OX-6-positive cells were located within the area of tyrosine hydroxylase (TH) -negativity. At 1 month after the lesion, the number of OX-6-positive cells was reduced in diet-treated animals while a significant increase beyond that observed at 1 week was now present in lesioned control animals. Dopamine recovery as revealed by TH-immunohistochemistry was significantly enhanced at 4 weeks postlesion in the striatum while in the globus pallidus the density of TH-positive nerve fibers was not different from control-fed lesioned animals. In conclusion, enhanced striatal dopamine recovery appeared in animals treated with diet enriched in antioxidants and anti-inflammatory phytochemicals and coincided with an early, transient increase in OX-6-positive microglia.
Place, publisher, year, edition, pages
2005. Vol. 196, no 2, 298-307 p.
Animals, Bacterial Proteins/*administration & dosage, Blueberry Plant, Calcium-Binding Proteins/metabolism, Cell Count/methods, Corpus Striatum/*cytology, Disease Models; Animal, Dopamine/*metabolism, Food; Formulated, Glial Fibrillary Acidic Protein/metabolism, Histocompatibility Antigens Class II/metabolism, Immunohistochemistry/methods, Male, Microglia/*physiology, Nerve Degeneration/chemically induced/*diet therapy/physiopathology, Neurons/physiology, Oxidopamine, Rats, Rats; Inbred F344, Recovery of Function/*physiology, Spirulina, Tyrosine 3-Monooxygenase/metabolism
IdentifiersURN: urn:nbn:se:umu:diva-12262DOI: doi:10.1016/j.expneurol.2005.08.013PubMedID: 16176814OAI: oai:DiVA.org:umu-12262DiVA: diva2:151933