Asaccharolytic anaerobic gram-negative coccobacilli (AAGNC) isolated from infected root canals and periodontal pockets.
2006 (English)In: Oral Microbiology and Immunology, Vol. 21, no 1, 28-31 p.Article in journal (Refereed) Published
BACKGROUND/AIMS: Culture-difficult bacteria, including asaccharolytic anaerobic gram-negative coccobacilli (AAGNC), may constitute a predominant group of organisms in oral sites. This study aimed to characterize phylogenetically 10 AAGNC isolated from endodontic lesions and periodontal pockets. METHODS: 16S rDNA sequence and G + C content were determined. Strains sharing more than 98% sequence similarities and similar G + C content were considered the same bacterial species. RESULTS: One isolate resembled Dialister pneumosintes (the type species of the genus Dialister) with 35 mol% G + C content and 97% sequence similarity. Of eight isolates having 45-47 mol% G + C content, seven were identified as D. invisus and one resembled Dialister invisus with 97% sequence similarity. However the 16S rDNA sequence similarities with D. pneumosintes were relatively low, indicating the strains may belong to a new genus. The last isolate revealed 35 mol% G + C content, but had higher 16S rDNA sequence similarity with D. invisus than with D. pneumosintes. CONCLUSION: The group of oral AAGNC isolates need to be reclassified
Place, publisher, year, edition, pages
2006. Vol. 21, no 1, 28-31 p.
IdentifiersURN: urn:nbn:se:umu:diva-12480OAI: oai:DiVA.org:umu-12480DiVA: diva2:152151