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Epoxy-Based Monoliths. A Novel Hydrophilic Separation Material for Liquid Chromatography of Biomolecules
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
2006 (English)In: Chemistry of Materials, Vol. 18, no 26, 6308-15 p.Article in journal (Refereed) Published
Abstract [en]

In our efforts to develop novel hydrophilic monolithic porous materials for use as supports in liquid chromatographic separation of proteins, polymers based on epoxy monomers and diamines as curing agents were synthesized. The epoxy dispersed phase was emulsified in an aqueous phase containing the amine with the aid of a nonionic polymeric surfactant, and the resulting emulsions were thermally polymerized. Various factors, namely, the type of epoxy component, levels of reactants, type and concentration of diluents, and curing procedures, were studied to obtain suitable morphology and adequate mechanical properties for their intended use. Characterization of their morphologies and porous properties was done using scanning electron microscopy, nitrogen adsorption/desorption measurement (BET method), mercury intrusion porosimetry, and X-ray photoelectron spectroscopy.

Place, publisher, year, edition, pages
2006. Vol. 18, no 26, 6308-15 p.
URN: urn:nbn:se:umu:diva-12507DOI: doi:10.1021/cm060645jOAI: diva2:152178
Available from: 2007-04-10 Created: 2007-04-10 Last updated: 2011-01-11Bibliographically approved
In thesis
1. New approaches to preparation of macroporous monoliths for use in liquid chromatography
Open this publication in new window or tab >>New approaches to preparation of macroporous monoliths for use in liquid chromatography
2009 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

High performance liquid chromatography (HPLC) is one of the major techniques in separat-ion sciences. Faster separation and higher efficiency are required to meet ever-growing demands. Despite numerous studies and achievements on improving mass transfer in particulate packings discontinuity seems to be the cornerstone drawback in their development. Macroporous continuous beds or monoliths are therefore a promising alternative to the particle medium. This thesis deals with preparation of new monoliths used as carrier for HPLC. Two different approaches were developed for two polymer systems. One was based on polycondensation of epoxy resins and polyamines which were components of an oil-in-water emulsion. An epoxy resin mixture was dispersed in aqueous polyamine phase with the aid of a surfactant. The other involved a traverse of a ready-made polymer solution around its upper critical solution temperature (UCST). In other words, linear polyamides, non-covalently crosslinked polymers, dissolved in a solvent at temperature higher than their UCST followed by slow cooling to below the critical temperature to precipitate the polymers. Partly re-established hydrogen bonds resulted in the formation of crystallites that interconnected into a network structure. Factors controlling morphology and porosity of final products were investigated. The study also deals with surface modifying for chromatographic applications. Functionalization pathways attempted in the thesis were quaterization of inherent amine of the epoxy-based monoliths and grafting tentacle ion groups via glycidyl methacrylate by atom transfer radical polymerization (ATRP) for ion exchange chromatography (IEC).

Place, publisher, year, edition, pages
Umeå: Umeå University, Department of Chemistry, 2009. 53 p.
monolith, polycondensation, dissolution-precipitation, epoxy-amine, poly-amide, nylon, emulsion polymerization, characterization, protein separation, liquid chromatography
National Category
Analytical Chemistry
Research subject
Analytical Chemistry
urn:nbn:se:umu:diva-20890 (URN)978-91-7264-772-5 (ISBN)
Kemiska institutionen, 90187, Umeå
Public defence
2009-04-17, KB3A9, Umeå University, Chemistry, Umeå, 13:00 (English)
Available from: 2009-03-30 Created: 2009-03-27 Last updated: 2014-03-10Bibliographically approved

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