Identification of c-Fos immunoreactive brainstem neurons activated during fictive mastication in the rabbit
2005 (English)In: Experimental Brain Research, ISSN 0014-4819, E-ISSN 1432-1106, Vol. 165, no 4, 478-489 p.Article in journal (Refereed) Published
In the present study we used the expression of the c-Fos-like protein as a "functional marker" to map populations of brainstem neurons involved in the generation of mastication. Experiments were conducted on urethane-anesthetized and paralyzed rabbits. In five animals (experimental group), rhythmical bouts of fictive masticatory-like motoneuron activity (cumulative duration 60-130 min) were induced by electrical stimulation of the left cortical "masticatory area" and recorded from the right digastric motoneuron pool. A control group of five animals (non-masticatory) were treated in the same way as the experimental animals with regard to surgical procedures, anesthesia, paralysis, and survival time. To detect the c-Fos-like protein, the animals were perfused, and the brainstems were cryosectioned and processed immunocytochemically. In the experimental group, the number of c-Fos-like immunoreactive neurons increased significantly in several brainstem areas. In rostral and lateral areas, increments occurred bilaterally in the borderzones surrounding the trigeminal motor nucleus (Regio h); the rostrodorsomedial half of the trigeminal main sensory nucleus; subnucleus oralis-gamma of the spinal trigeminal tract; nuclei reticularis parvocellularis pars alpha and nucleus reticularis pontis caudalis (RPc) pars alpha. Further caudally-enhanced labeling occurred bilaterally in nucleus reticularis parvocellularis and nucleus reticularis gigantocellularis (Rgc) including its pars-alpha. Our results provide a detailed anatomical record of neuronal populations that are correlated with the generation of the masticatory motor behavior.
Place, publisher, year, edition, pages
2005. Vol. 165, no 4, 478-489 p.
Anesthesia, Animals, Brain Stem/*metabolism, Data Interpretation; Statistical, Efferent Pathways/cytology/physiology, Electric Stimulation, Immunohistochemistry, Male, Mastication/*physiology, Masticatory Muscles/innervation/physiology, Microelectrodes, Motor Cortex/physiology, Motor Neurons/physiology, Neurons/*metabolism, Paralysis/physiopathology, Proto-Oncogene Proteins c-fos/*metabolism, Rabbits
IdentifiersURN: urn:nbn:se:umu:diva-12592DOI: 10.1007/s00221-005-2319-5PubMedID: 15887006OAI: oai:DiVA.org:umu-12592DiVA: diva2:152263