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Improved organotypic cell culture model for analysis of the neuronal circuit involved in the monosynaptic stretch reflex.
Umeå University, Faculty of Medicine, Integrative Medical Biology, Physiology.
Umeå University, Faculty of Medicine, Integrative Medical Biology, Physiology.
2006 (English)In: Journal of Neuroscience Research, ISSN 0360-4012, Vol. 84, no 2, 460-9 p.Article in journal (Refereed) Published
Abstract [en]

Knowledge regarding neuronal circuit formation is central for the understanding of the vast network making up the brain. It is therefore necessary to find novel ways to analyze the mechanisms involved in well-defined neural circuits. We present an improved in vitro model of the monosynaptic stretch reflex circuit, based on primary organotypic cell cultures. By using limb tissue as a source of muscle fibers instead of circumspinal tissue we could make the in vitro system more in vivo like in the sense that it focuses on the stretch reflex involving limb muscles. Furthermore, our analyses showed that this procedure allows muscle fibers to follow the normal developmental pattern. Particularly interesting was the finding of slow tonic myosin heavy chain expressing muscle fibers, a developmental marker for muscle spindles, in the cultures showing that this system has the potential to contain the complete reflex circuits.

Place, publisher, year, edition, pages
2006. Vol. 84, no 2, 460-9 p.
Keyword [en]
Animals, Coculture Techniques, Electric Stimulation, Ganglia; Spinal/physiology, Immunohistochemistry, Mice, Mice; Inbred BALB C, Models; Neurological, Motor Neurons/physiology, Muscle; Skeletal/physiology, Nerve Net/*physiology, Neurons; Afferent/physiology, Organ Culture Techniques/*methods, Rats, Rats; Wistar, Reflex; Monosynaptic/*physiology, Reflex; Stretch/*physiology, Spinal Cord/physiology
URN: urn:nbn:se:umu:diva-12645DOI: doi:10.1002/jnr.20888PubMedID: 16683231OAI: diva2:152316
Available from: 2008-01-11 Created: 2008-01-11 Last updated: 2011-01-11Bibliographically approved

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