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Alginate hydrogel and matrigel as potential cell carriers for neurotransplantation.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy.
Umeå University, Faculty of Medicine, Department of Integrative Medical Biology (IMB), Anatomy. Umeå University, Faculty of Medicine, Department of Surgical and Perioperative Sciences, Hand Surgery.
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2006 (English)In: Journal of Biomedical Materials Research part A, ISSN 1549-3296, Vol. 77, no 2, 242-252 p.Article in journal (Refereed) Published
Abstract [en]

Development of biosynthetic conduits carrying extracellular matrix molecules and cell lines expressing neurotrophic growth factors represents a novel and promising strategy for spinal cord and peripheral nerve repair. In the present in vitro study, the compatibility and growth-promoting effects of (i) alginate hydrogel, (ii) alginate hydrogel complemented with fibronectin, and (iii) matrigel were compared between olfactory ensheathing cells (OECs), Schwann cells (SCs), and bone marrow stromal cells (BMSCs). Neurite outgrowth from embryonic dorsal root ganglia (DRG) neurons was used to assess the efficacy of the hydrogels alone or in combination with cultured cells to promote axonal regeneration. The result showed that alginate hydrogel transformed OECs, SCs, and BMSCs into atypical cells with spherical shape and inhibited their metabolic activity. Combination of alginate hydrogel with fibronectin promoted only OECs proliferation. Alginate hydrogel also inhibited outgrowth of DRG neurites, although this effect was attenuated by addition of fibronectin, SCs, or BMSCs. In contrast, matrigel stimulated cell proliferation, preserved the typical morphological features of the cultured cells and induced massive sprouting of DRG neurites. Addition of cultured cells to matrigel did not further improve DRG neurite outgrowth. The present findings suggest that addition of extracellular matrix should be considered when engineering biosynthetic scaffolds on the basis of alginate hydrogels.

Place, publisher, year, edition, pages
2006. Vol. 77, no 2, 242-252 p.
Keyword [en]
Alginates/chemistry/metabolism, Animals, Animals; Newborn, Biocompatible Materials/chemistry/metabolism, Bone Marrow Cells/cytology/metabolism, Cell Shape, Cell Transplantation/*methods, Cells; Cultured, Collagen/chemistry/metabolism, Drug Combinations, Female, Fibronectins/metabolism, Ganglia; Spinal/cytology, Hydrogels/chemistry/metabolism, Laminin/chemistry/metabolism, Materials Testing, Nerve Regeneration/*physiology, Neurites/metabolism/ultrastructure, Olfactory Bulb/cytology, Proteoglycans/chemistry/metabolism, Rats, Rats; Sprague-Dawley, Schwann Cells/cytology/metabolism/transplantation, Stromal Cells/cytology/metabolism/transplantation
URN: urn:nbn:se:umu:diva-12722DOI: doi:10.1002/jbm.a.30603PubMedID: 16392134OAI: diva2:152393
Available from: 2007-12-11 Created: 2007-12-11 Last updated: 2010-06-24Bibliographically approved

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