Change search
ReferencesLink to record
Permanent link

Direct link
Hydrophilic interaction chromatography
Umeå University, Faculty of Science and Technology, Chemistry.
Umeå University, Faculty of Science and Technology, Chemistry.
2006 (English)In: Journal of Separation Science, Vol. 29, 1784-1821 p.Article in journal (Refereed) Published
Abstract [en]

Separation of polar compounds on polar stationary phases with partly aqueous eluents is by no means a new separation mode in LC. The first HPLC applications were published more than 30 years ago, and were for a long time mostly confined to carbohydrate analysis. In the early 1990s new phases started to emerge, and the practice was given a name, hydrophilic interaction chromatography (HILIC). Although the use of this separation mode has been relatively limited, we have seen a sudden increase in popularity over the last few years, promoted by the need to analyze polar compounds in increasingly complex mixtures. Another reason for the increase in popularity is the widespread use of MS coupled to LC. The partly aqueous eluents high in ACN with a limited need of adding salt is almost ideal for ESI. The applications now encompass most categories of polar compounds, charged as well as uncharged, although HILIC is particularly well suited for solutes lacking charge where coulombic interactions cannot be used to mediate retention. The review attempts to summarize the ongoing discussion on the separation mechanism and gives an overview of the stationary phases used and the applications addressed with this separation mode in LC.

Place, publisher, year, edition, pages
2006. Vol. 29, 1784-1821 p.
Keyword [en]
Aqueous normal phase, Chromatography, Hydrophilic interaction chromatography, Hydrophilic interaction, Water
URN: urn:nbn:se:umu:diva-12872DOI: doi:10.1002/jssc.200600199OAI: diva2:152543
Available from: 2007-04-23 Created: 2007-04-23 Last updated: 2011-01-11Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Hemström, PetrusIrgum, Knut
By organisation

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 53 hits
ReferencesLink to record
Permanent link

Direct link