Molecularly imprinted polymers grafted to flow through poly(trimethylolpropane trimethacrylate) monoliths for capillary-based solid-phase extraction
2006 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1109, no 1, 92-99 p.Article in journal (Refereed) Published
Monolithic previous termmolecularly imprinted polymersnext term (mMIPs) have been synthesized in a novel way using a trimethylolpropane trimethacrylate core material photo-polymerized in situ in a 100 μm I.D. UV-transparent capillary and further photo-previous termgraftednext term to create specific cavities in the previous termgraftednext term layer. This polymerization technique allows the imprints to be directly created on the surface of the material using a minimum amount of template. Three different anaesthetics of similar structures (bupivacaine, mepivacaine and S-ropivacaine) were used as model target molecules to synthesize sample enrichment media. Hence, various mMIPs have been prepared and evaluated on a micro-system against each analyte in order to test the retention properties and cross-selectivities of the materials. The retention factors were determined and compared with the non-previous termimprintednext term reference column (mNIP), yielding high imprinting factors together with good selectivity factors between the three analytes. A study with a pure enantiomeric target was carried out to assess the degree of stereo-specific imprinting for injection of racemic mixtures. Finally, one column was previous termimprintednext term with an equimolar mixture of all three anaesthetics to provide further comprehension of the retention mechanism and accredit the possibility of using the material as a sample enrichment entity. Scanning electron microscopy (SEM), nitrogen absorption/desorption (BET) and mercury intrusion porosimetry were used to characterize the monolith and the mMIPs properties. Nuclear magnetic resonance (NMR) has been used to assess the similarities between the mMIP and mNIP.
Place, publisher, year, edition, pages
Amsterdam: Elsevier , 2006. Vol. 1109, no 1, 92-99 p.
previous termMolecularly imprinted polymernext term, Bupivacaine, Monolith, Capillary, Liquid chromatography
IdentifiersURN: urn:nbn:se:umu:diva-12904DOI: doi:10.1016/j.chroma.2005.12.014OAI: oai:DiVA.org:umu-12904DiVA: diva2:152575