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Extending the array of crosslinkers suitable for the preparation of polymethacrylate-based monoliths
Umeå University, Faculty of Science and Technology, Chemistry.
Department of Chemistry, University of California, Berkeley, CA, USA.
Department of Chemistry, University of California, Berkeley, CA, USA.
Umeå University, Faculty of Science and Technology, Chemistry.
2005 (English)In: Journal of Separation Science, ISSN 1615-9306, Vol. 28, no 17, 2401-2406 p.Article in journal (Refereed) Published
Abstract [en]

The in situ preparation of monolithic capillary columns comprising copolymers of butyl methacrylate with ethylene dimethacrylate, diethylene glycol dimethacrylate, triethylene glycol dimethacrylate, and pentaerythritol tetraacrylate using thermal polymerization within 250 mu m ID capillaries and their application for mu-HPLC separations of proteins has been studied. For all crosslinkers, optimization of the porogenic mixture consisting of 1-propanol and 1,4-butanediol yielded monoliths with pore sizes above 1 mu m suitable for rapid separations at low back pressure. Very good separations were achieved for a protein mixture consisting of ribonuclease A, cytochrome c, myoglobin, and ovalbumin with all tested columns.

Place, publisher, year, edition, pages
2005. Vol. 28, no 17, 2401-2406 p.
Keyword [en]
capillary, crosslinker, mu-HPLC, monolith, monolithic column, polymerization, pore size, protein separation, reverse phase
URN: urn:nbn:se:umu:diva-13015DOI: 10.1002/jssc.200500222OAI: diva2:152686
Available from: 2007-04-27 Created: 2007-04-27 Last updated: 2009-06-29Bibliographically approved
In thesis
1. Synthesis and modifications of materials for separation science
Open this publication in new window or tab >>Synthesis and modifications of materials for separation science
2008 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

This thesis deals with the preparation of materials for use in separation science and their surface modification by grafting. The overall aim is the preparation of diverse materials by combination of a set of developed tools.

Included in the thesis is the synthesis of monolithic media using non-traditional crosslinkers, the characterization of their porous properties and initial testing in reversed-phase chromatographic separation of proteins. The preparation of a library of short polymer chains, telomers, with varied functionality and their characterization is reported. Included in the characterization is the gradient polymer elution chromatography of selected telomers on a monolithic column in capillary format. The technique shows promise as a tool for monitoring of polymerization processes and for the separation of telomers with similar size but different functionalities or characteristics.

Finally, the combination of polymeric support materials and the prepared telomer library is used in surface modification. Surface modification is performed onto activated surfaces via a “grafting to” approach. One example is shown, the surface modification of epoxy-modified divinylbenzene particles by attachment of telomer chains introducing ion-exchange functionality. The material is tested for the separation of proteins, in ion-exchange chromatography mode.

Place, publisher, year, edition, pages
Umeå: Kemi, 2008. 43 p.
material synthesis, monolith, surface modification, telomer, “grafting to”, gradient polymer elution, chromatography
National Category
Other Basic Medicine
urn:nbn:se:umu:diva-1572 (URN)978-91-7264-528-8 (ISBN)
Public defence
2008-03-28, N360, Naturvetarhuset, Umeå Universitet, Umeå, 10:00 (English)
Available from: 2008-03-07 Created: 2008-03-07 Last updated: 2010-01-21Bibliographically approved

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