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Two-photon excited fluorescence depolarisation experiments: II. The proper response function for analysing TCSPC data
Umeå University, Faculty of Science and Technology, Chemistry.
2005 (English)In: CHEMICAL PHYSICS LETTERS, ISSN 0009-2614, Vol. 411, no 1-3, 51-60 p.Article in journal (Refereed) Published
Abstract [en]

The instrumental response function (IRF) needed for the analyses of fluorescence time-correlated single-photon counting (TCSPC) experiments is usually determined by measuring the Rayleigh scattering (RS) of colloidal particles. Since RS is a one-photon process the response function obtained is referred to as a one-photon IRF (OP-IRF). Hence, in a two-photon excited (TPE) fluorescence experiment the hyper-Rayleigh scattering (HRS) might suit the determination of the two-photon IRF better [A. Habenicht, J. Hjelm, E. Mukhtar, F. Bergstrom, L.B.-angstrom. Johansson, Chem. Phys. Lett. 354 (2002) 3671.

In the present study, we explore the importance of OP- and TP-IRF in fluorescence depolarisation studies. POPOP in ethanol and n-octanol is analysed when using OP- and TP-IRF. It is demonstrated that the TP-IRF yields significantly better statistics.

Place, publisher, year, edition, pages
2005. Vol. 411, no 1-3, 51-60 p.
Keyword [en]
HYPER-RAYLEIGH SCATTERING, ROTATIONAL DIFFUSION, ANISOTROPY DECAY, SPECTROSCOPY, SINGLE, RELAXATION, EXCITATION, DYNAMICS, LIQUIDS, PROBE
Identifiers
URN: urn:nbn:se:umu:diva-13247DOI: doi:10.1016/j.cplett.2005.05.121OAI: oai:DiVA.org:umu-13247DiVA: diva2:152918
Available from: 2007-06-26 Created: 2007-06-26 Last updated: 2011-01-12Bibliographically approved

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  • apa
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